GUT VIrus BRain Axis Technology In OrgaNoid Science

Project Information

GUTVIBRATIONS

Grant agreement ID: 953201

DOI 10.3030/953201

Project closed

EC signature date 18 February 2021

Start date 1 April 2021

End date 31 March 2025

Funded under

• INDUSTRIAL LEADERSHIP - Leadership in enabling and industrial technologies – Nanotechnologies

Total cost No data

EU contribution € 6 061 375,00

Coordinated by

STICHTING AMSTERDAM UMC
Netherlands

CORDIS provides links to public deliverables and publications of HORIZON projects.

Links to deliverables and publications from FP7 projects, as well as links to some specific result types such as dataset and software, are dynamically retrieved from OpenAIRE .

Deliverables

Documents, reports (11)

Protocol for establishing the gut-epithelial model in STACKS [M6]. A standard protocol for the transfer of gut epithelial monolayer model from Transwell to STACKS will be provided. [R] [PU]

Protocol for establishing the gutepithelial model in STACKS M6 A standard protocol for the transfer of gut epithelial monolayer model from Transwell to STACKS will be provided R PU

Report on diffusion properties through the Biosilk matrix [M12]. Diffusion properties of fluorochromes in the Biosilk network with and without cells will be reported. [R] [PU]

Report on diffusion properties through the Biosilk matrix M12 Diffusion properties of fluorochromes in the Biosilk network with and without cells will be reported R PU

Definition of standard values for specific immune response parameters in the gut epithelial-immune cell co-culture model [M24]. A report on the measured standard values for immune markers will be available. [R] [PU]
Manuscript on the effect of fibroblasts on gut epithelial differentiation [M24]. The role of fibroblasts within the Biosilk network to effect gut differentiation in vitro will be elucidated. [R] [PU]
Protocol for cytotoxicity assay in gut mucosa model [M30] [Report on the best method for cytotoxicity assessment] [R] [PU]
Protocol for iPSC-derived BBB model [M24] [A standard protocol for establishing a BBB model will be provided]. [R] [PU]
Manuscript on the method to co-culture gut-epithelial model with immune cells [M24]. [R] [PU]
Report on the properties of the Biosilk network [M24]. The complete properties of the Biosilk network such as tensile and cell motility will be characterised. [R] [PU]
Characterisation panel for iPSC-derived BBB model. [M24] [Report on standard range of parameters for BBB model] [R] [PU]
Report on the properties of the Biosilk nanomembrane [M24]. The complete properties of the Biosilk nanomembrane such as porosity and mass transport will be characterised. [R] [PU]
"Protocol for patterning ""crypt-villi"" features on Biosilk [M9]. A standard protocol for embossing or casting ""crypt-villi"" structures will be delivered. [R] [PU]"

Protocol for patterning cryptvilli features on Biosilk M9 A standard protocol for embossing or casting cryptvilli structures will be delivered R PU

Demonstrators, pilots, prototypes (1)

Demonstrator of BBB-brain model [M36] A co-culture of BBB-brain is established [DEM] [PU]

Open Research Data Pilot (1)

Open Research Data Pilot

Other (1)

GUTVIBRATIONS Website [M1]. Public release of the website [OTHER] [PU]

Publications

Peer reviewed articles (16)

What is a neurotropic virus: Discrepancies in terminology between clinical and basic science

Author(s): Pamela E. Capendale, Katja C. Wolthers, Dasja Pajkrt
Published in: Med, Issue 4, 2024, Page(s) 660-663, ISSN 2666-6340
Publisher: Elsevier BV
DOI: 10.1016/j.medj.2023.07.001

A quantitative meta-analysis: Is organ on a chip cell cultures better than static cell cultures?

Author(s): Martin Dufva
Published in: Scientific Reports, 2023, ISSN 2045-2322
Publisher: Nature Publishing Group
DOI: 10.21203/rs.3.rs-2208798/v1

Parechovirus infection in human brain organoids: host innate inflammatory response and not neuro-infectivity correlates to neurologic disease

Author(s): Pamela E. Capendale, Inés García-Rodríguez, Anoop T. Ambikan, Lance A. Mulder, Josse A. Depla, Eline Freeze, Gerrit Koen, Carlemi Calitz, Vikas Sood, Renata Vieira de Sá, Ujjwal Neogi, Dasja Pajkrt, Adithya Sridhar, Katja C. Wolthers
Published in: Nature Communications, Issue 15, 2024, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-024-46634-9

Non-Polio Enterovirus C Replicate in Both Airway and Intestine Organotypic Cultures

Author(s): Giulia Moreni, Hetty van Eijk, Gerrit Koen, Nina Johannesson, Carlemi Calitz, Kimberley Benschop, Jeroen Cremer, Dasja Pajkrt, Adithya Sridhar, Katja Wolthers
Published in: Viruses, Issue 15, 2025, Page(s) 1823, ISSN 1999-4915
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/v15091823

Tissue Like Density Sheets of Hepatoblastoma Cultures Obtained with Thin Suspended Hydrogels Mimicking In Vivo Inter‐Capillary Distances

Author(s): Alexander Jönsson, Hakan Gürbüz, Louise Wildfang, Pernille Rose Jensen, Martin Dufva
Published in: Advanced Materials Technologies, 2024, ISSN 2365-709X
Publisher: Advanced Materials
DOI: 10.1002/admt.202401204

Advances in Animal Models and Cutting-Edge Research in Alternatives: Proceedings of the Third International Conference on 3Rs Research and Progress, Vishakhapatnam, 2022

Author(s): Nikita Narayan Naik, Bharadwaja Vadloori, Suresh Poosala, Pratima Srivastava, Sandra Coecke, Adrian Smith, Aysha Akhtar, Clive Roper, Sridhar Radhakrishnan, Balaji Bhyravbhatla, Madhujit Damle, Venkat Koushik Pulla, Johannes Hackethal, Reyk Horland, Albert P. Li, Falguni Pati, Manu Smriti Singh, Paola Occhetta, Rohit Bisht, Prajakta Dandekar, Krishna Bhagavatula, Dasja Pajkrt, Michael Johnson, Til
Published in: Alternatives to Laboratory Animals, Issue 51, 2025, Page(s) 263-288, ISSN 0261-1929
Publisher: Fund for the Replacement of Animals in Medical Experiments
DOI: 10.1177/02611929231180428

Assessment of the broad-spectrum host targeting antiviral efficacy of halofuginone hydrobromide in human airway, intestinal and brain organotypic models.

Author(s): Inés García-Rodríguez, Giulia Moreni, Pamela E. Capendale, Lance Mulder, Ikrame Aknouch, Renata Vieira de Sá, Nina Johannesson, Eline Freeze, Hetty van Eijk, Gerrit Koen, Katja C. Wolthers, Dasja Pajkrt, Adithya Sridhar, Carlemi Calitz
Published in: Antiviral Research, Issue 222, 2024, Page(s) 105798, ISSN 0166-3542
Publisher: Elsevier BV
DOI: 10.1016/j.antiviral.2024.105798

Enhanced biocompatibility of 3D printed resin parts <i>via</i> wet autoclave postprocessing: implications for stem cell organ-on-a-chip culture

Author(s): Alexander Jönsson, Antonia Iatrou, Louise Wildfang, Dana J. Neumann, Hakan Gürbüz, Carina A. A. Schoenmaker, Marlene Danner Dalgaard, Pernille Rose Jensen, Martin Dufva
Published in: Materials Advances, 2025, ISSN 2633-5409
Publisher: Materials Advances
DOI: 10.1039/d4ma01191k

A beginner’s guide on the use of brain organoids for neuroscientists: a systematic review

Author(s): Lance A. Mulder, Josse A. Depla, Adithya Sridhar, Katja Wolthers, Dasja Pajkrt, Renata Vieira de Sá
Published in: Stem Cell Research &amp; Therapy, Issue 14, 2023, ISSN 1757-6512
Publisher: BioMed Central
DOI: 10.1186/s13287-023-03302-x

Editorial: Human organoid technology for virus research

Author(s): Dasja Pajkrt, Veronica Krenn, Joana Rocha-Pereira
Published in: Frontiers in Cellular and Infection Microbiology, Issue 13, 2023, ISSN 2235-2988
Publisher: Frontiers Media S. A.
DOI: 10.3389/fcimb.2023.1155252

Understanding neurotropic enteric viruses: routes of infection and mechanisms of attenuation

Author(s): Valeria Lulla, Adithya Sridhar
Published in: Cellular and Molecular Life Sciences, Issue 81, 2024, ISSN 1420-682X
Publisher: Birkhauser Verlag
DOI: 10.1007/s00018-024-05450-6

A quantitative meta-analysis comparing cell models in perfused organ on a chip with static cell cultures

Author(s): Martin Dufva
Published in: Scientific Reports, Issue 13, 2023, ISSN 2045-2322
Publisher: Nature Publishing Group
DOI: 10.1038/s41598-023-35043-5

Evaluation of 3D Human Intestinal Organoids as a Platform for EV-A71 Antiviral Drug Discovery

Author(s): Fatma Masmoudi, Nanci Santos-Ferreira, Dasja Pajkrt, Katja C. Wolthers, Jeroen DeGroot, Maria L. H. Vlaming, Joana Rocha-Pereira, Ludovico Buti
Published in: Cells, Issue 12, 2023, Page(s) 1138, ISSN 2073-4409
Publisher: MDPI AG
DOI: 10.3390/cells12081138

Current and Future Antiviral Strategies to Tackle Gastrointestinal Viral Infections

Author(s): Nanci Santos-Ferreira, Jana Van Dycke, Johan Neyts, Joana Rocha-Pereira
Published in: Microorganisms, Issue 9, 2024, Page(s) 1599, ISSN 2076-2607
Publisher: Microorganisms
DOI: 10.3390/microorganisms9081599

Human milk inhibits some enveloped virus infections, including SARS-CoV-2, in an intestinal model

Author(s): Ikrame Aknouch, Adithya Sridhar, Eline Freeze, Francesca Paola Giugliano, Britt J van Keulen, Michelle Romijn, Carlemi Calitz, Inés García-Rodríguez, Lance Mulder, Manon E Wildenberg, Vanesa Muncan, Marit J van Gils, Johannes B van Goudoever, Koert J Stittelaar, Katja C Wolthers, Dasja Pajkrt
Published in: Life Science Alliance, Issue 5, 2024, Page(s) e202201432, ISSN 2575-1077
Publisher: Frontiers
DOI: 10.26508/lsa.202201432

Amino acid variation at VP1-145 of enterovirus A71 determines the viral infectivity and receptor usage in a primary human intestinal model

Author(s): Ikrame Aknouch, Inés García-Rodríguez, Francesca Paola Giugliano, Carlemi Calitz, Gerrit Koen, Hetty van Eijk, Nina Johannessson, Sjoerd Rebers, Lieke Brouwer, Vanesa Muncan, Koert J. Stittelaar, Dasja Pajkrt, Katja C. Wolthers, Adithya Sridhar
Published in: Frontiers in Microbiology, Issue 14, 2023, ISSN 1664-302X
Publisher: Frontiers Media
DOI: 10.3389/fmicb.2023.1045587