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LONGSENSE - A novel biosensor for lncRNA

Project description

Using long non-coding RNAs as diagnostic biomarkers

Accumulating evidence underscores various important biological roles for non-coding RNAs (nc-RNAs). RNAs with a length of more than 200 nucleotides, the long non-coding RNAs (lncRNAs), can potentially serve as diagnostic biomarkers as their aberrant expression has been linked to various neurodegenerative and psychiatric diseases, diabetes and cancer. However, current technologies for lncRNA isolation and detection are laborious and hence unsuitable for routine diagnosis. Employing computer modelling for molecular reactions and RNA structure, scientists of the EU-funded LONGSENSE project aim to develop a novel biosensor for lncRNA detection both for research and diagnostic purposes.

Objective

The function of ribonucleic acid (RNA) was long thought to be only an intermediate product in the protein synthesis machinery, but this view changed since the biological significance of the non-coding protein fraction of RNA was discovered. More recently a still evolving class of RNAs with a length above 200 nucleotides, the long non-coding RNAs (lncRNA) came into the spotlight. Up- or downregulation have been associated with human diseases, such as neurodegenerative and psychiatric diseases, cardiac diseases, diabetes as well as cancer, offering the possibility to use lncRNA as biomarker for diagnosis and prognosis. The currently used method for lncRNA detection is complicated and only a reliable method for an experienced researcher with the necessary lab facilities. Detection of lncRNA follows a time-consuming protocol, taking at least 1 day from sample preparation to analysed data. To exploit the full potential of lncRNA as new biomarker for several diseases, the detection method needs to be simplified. This would allow to use lncRNA as biomarker on-spot in private praxis and integrate lncRNA in the routine diagnostic of clinics. LONGSENSE will develop a fast, user-friendly and cost-effective biosensor for lncRNA detection. The use of computer modelling for molecular reactions and RNA structure, will enable us to work efficiently and remain flexible to the rapid development in the scientific field and adapt a product to meet the needs of the market. The aims of the project are to (1) create a multi-level biosensor for the detection of disease relevant lncRNA in well-plate format for high throughput screening to be used in clinical labs or research; (2) design a device to be used on the spot; (3) asses the commercial feasibility of the technology.

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Topic(s)

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Funding Scheme

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ERC-POC-LS - ERC Proof of Concept Lump Sum Pilot

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Call for proposal

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(opens in new window) ERC-2020-PoC

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Host institution

TECHNISCHE UNIVERSITAT DARMSTADT
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 150 000,00
Address
KAROLINENPLATZ 5
64289 DARMSTADT
Germany

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Region
Hessen Darmstadt Darmstadt, Kreisfreie Stadt
Activity type
Higher or Secondary Education Establishments
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Total cost

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Beneficiaries (1)

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