AN IN VIVO BIOENGINEERED CHIP AS A SMART INTRAVITAL MULTIPHOTON IMAGING WINDOW FOR NEW VALIDATION PROTOCOLS OF BIOMATERIALS

Project Information

IN2SIGHT

Grant agreement ID: 964481

DOI

10.3030/964481

Project closed

EC signature date 21 December 2020

Start date 1 March 2021

End date 31 August 2025

Funded under

• EXCELLENT SCIENCE - Future and Emerging Technologies (FET)

Total cost

€ 3 438 959,00

EU contribution

€ 3 438 959,00

Coordinated by

UNIVERSITA' DEGLI STUDI DI MILANO-BICOCCA
Italy

CORDIS provides links to public deliverables and publications of HORIZON projects.

Links to deliverables and publications from FP7 projects, as well as links to some specific result types such as dataset and software, are dynamically retrieved from OpenAIRE .

Deliverables

Documents, reports (11)

Report on the design of IN2SIGHT, atlas and microlenses

Report on the design of IN2SIGHT atlas and lenses

Protocols for chip implantation and in vivo imaging
Report on the 2PP structuring of the IN2SIGHT
Regulatory Action Plan
Hybrid Nanolithography-2PP fabrication

Hybrid Nanolithography2PP fabrication

Report of immune reaction to INSIGHT
Simulation requirements & design report

Simulation requirements design report

Risk assessment report - M24

Risk assessment report

Risk assessment report - M12

Risk assessment report

Immunological and device modelling report
DMP report

Publications

Peer reviewed articles (21)

CollagenFitJ, a FIJI plugin for the quantification of collagen in polarization-resolved second harmonic generation image sets

Author(s): Radu Hristu, Farzad Fereidouni, Stefan G. Stanciu, Lucian G. Eftimie, Oana C. Voinea, Danielis Rutkauskas, Laura Sironi, Giuseppe Chirico, Denis E. Tranca, Remus R. Glogojeanu, Alberto Diaspro, George A. Stanciu
Published in: Advanced Photonics Nexus, Issue 4, 2025, Page(s) Vol. 4, Issue 3, 037001, ISSN 2791-1519
Publisher: SPIE-Intl Soc Optical Eng
DOI: 10.1117/1.apn.4.3.037001

Neural Precursor Cells Expanded Inside the 3D Micro-Scaffold Nichoid Present Different Non-Coding RNAs Profiles and Transcript Isoforms Expression: Possible Epigenetic Modulation by 3D Growth

Author(s): Letizia Messa, Bianca Barzaghini, Federica Rey, Cecilia Pandini, Gian Vincenzo Zuccotti, Cristina Cereda, Stephana Carelli Carelli, Manuela Teresa Raimondi
Published in: Biomedicines, Issue 9, 2025, Page(s) 1120, ISSN 2227-9059
Publisher: MDPI AG
DOI: 10.3390/biomedicines9091120

A Miniaturized Imaging Window to Quantify Intravital Tissue Regeneration within a 3D Microscaffold in Longitudinal Studies

Author(s): Claudio Conci, Emanuela Jacchetti, Laura Sironi, Lorenzo Gentili, Giulio Cerullo, Roberto Osellame, Giuseppe Chirico, Manuela Teresa Raimondi
Published in: Advanced Optical Materials, Issue 2101103, 2022, Page(s) 1-12, ISSN 2195-1071
Publisher: Wiley online
DOI: 10.1002/adom.202101103

Proteinaceous microstructure in a capillary: a study of non-linear bending dynamics

Author(s): Marini, M., Zeynali, A., Collini, M., Bouzin M, Sironi L., D'Alfonso L., Mantegazza F., Cassina, V., Chirico, G.
Published in: Lab on a chip, Issue 22, 2022, Page(s) 4917-4932, ISSN 1473-0197
Publisher: Royal Society of Chemistry
DOI: 10.1039/d2lc00697a

Melanin concentration maps by label-free super-resolution photo-thermal imaging on melanoma biopsies

Author(s): Margaux Bouzin, Mario Marini, Giuseppe Chirico, Francesca Granucci, Francesca Mingozzi, Roberto Colombo, Laura D’Alfonso, Laura Sironi, Maddalena Collini
Published in: Biomedical Optics Express, Issue 13, 2024, Page(s) 1173, ISSN 2156-7085
Publisher: The Optical Society
DOI: 10.1364/boe.445945

Effect of 3D Synthetic Microscaffold Nichoid on the Morphology of Cultured Hippocampal Neurons and Astrocytes

Author(s): Clara Alice Musi, Luca Colnaghi, Arianna Giani, Erica Cecilia Priori, Giacomo Marchini, Matteo Tironi, Claudio Conci, Giulio Cerullo, Roberto Osellame, Manuela Teresa Raimondi, Andrea Remuzzi, Tiziana Borsello
Published in: Cells, Issue 11, 2025, Page(s) 2008, ISSN 2073-4409
Publisher: MDPI AG
DOI: 10.3390/cells11132008

<i>In vivo</i> label-free tissue histology through a microstructured imaging window

Author(s): Claudio Conci, Laura Sironi, Emanuela Jacchetti, Davide Panzeri, Donato Inverso, Rebeca Martínez Vázquez, Roberto Osellame, Maddalena Collini, Giulio Cerullo, Giuseppe Chirico, Manuela Teresa Raimondi
Published in: APL Bioengineering, Issue 8, 2025, Page(s) 016102, ISSN 2473-2877
Publisher: AIP Publishing
DOI: 10.1063/5.0165411

Understanding local immunity to enable regionalized medicine

Author(s): Marco De Giovanni, Donato Inverso, Matteo Iannacone
Published in: The EMBO Journal, Issue 43, 2024, Page(s) 5788-5792, ISSN 1460-2075
Publisher: Springer Science and Business Media LLC
DOI: 10.1038/s44318-024-00255-6

Development of an Oriented Co-Culture System Using 3D Scaffolds Fabricated via Non-Linear Lithography.

Author(s): Kordas, A., Manganas, P., Selimis, A., Barmparis, G. D., Farsari, M., & Ranella, A.
Published in: Materials, 2022, Page(s) 15(12), ISSN 1996-1944
Publisher: MDPI Open Access Publishing
DOI: 10.3390/ma15124349

Quantitative active super-resolution thermal imaging: The melanoma case study

Author(s): Mario Marini, Margaux Bouzin, Riccardo Scodellaro, Laura D’Alfonso, Laura Sironi, Francesca Granucci, Francesca Mingozzi, Giuseppe Chirico, Maddalena Collini
Published in: Biomolecular Concepts, Issue 13, 2022, Page(s) 242-255, ISSN 1868-503X
Publisher: Walter de Gruyter GmbH
DOI: 10.1515/bmc-2022-0015

3D photopolymerized microstructured scaffolds influence nuclear deformation, nucleo/cytoskeletal protein organization, and gene regulation in mesenchymal stem cells

Author(s): Francesca Donnaloja, Manuela Teresa Raimondi, Letizia Messa, Bianca Barzaghini, Federica Carnevali, Emanuele Colombo, Davide Mazza, Chiara Martinelli, Lucia Boeri, Federica Rey, Cristina Cereda, Roberto Osellame, Giulio Cerullo, Stephana Carelli, Monica Soncini, Emanuela Jacchetti
Published in: APL Bioengineering, Issue 7, 2025, Page(s) 036112, ISSN 2473-2877
Publisher: AIP Publishing
DOI: 10.1063/5.0153215

Implantable Micro-optics for label-free non-linear imaging

Author(s): Mario Marini, Davide Panzeri, Alessandra Nardini, Behjat S. Kariman, Claudio Conci, Laura Sironi, Donato Inverso, Emanuela Jacchetti, Rebeca Martinez Vázquez, Roberto Osellame, Maddalena Collini, Giulio Cerullo, Manuela Teresa Raimondi, Elmina Kabouraki, Maria Farsari, Nikos Kehaghias, Konstantina Tourlouki, Giuseppe Chirico
Published in: EPJ Web of Conferences, Issue 309, 2024, Page(s) 04011, ISSN 2100-014X
Publisher: EDP Sciences
DOI: 10.1051/epjconf/202430904011

Multiphoton laser fabrication of hybrid photo-activable biomaterials

Author(s): Margaux Bouzin; Amirbahador Zeynali; Mario Marini; Laura Sironi; Riccardo Scodellaro; Laura D'Alfonso; Maddalena Collini; Giuseppe Chirico
Published in: Sensors (Basel, Switzerland), Issue 1, 2021, ISSN 1424-8220
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/s21175891

Fabrication of Plano-convex Microlenses using Two-Photon Polymerization for Bioimaging with Non-Linear Excitation Microscopy

Author(s): Behjat S. Kariman, Alessandra Nardini, Marco Grassi, Mario Marini, Claudio Conci, Margaux Bouzin, Maddalena Collini, Manuela T. Raimondi, Giuseppe Chirico, Roberto Osellame, Giulio Cerullo, Rebeca Martínez Vázquez
Published in: EPJ Web of Conferences, Issue 287, 2023, Page(s) 03009, ISSN 2100-014X
Publisher: EDP Sciences
DOI: 10.1051/epjconf/202328703009

Engineering macrophage responses through 3D scaffold microarchitecture

Author(s): Chiara Martinelli, Srijan Chakraborty, Giovanni Buccioli, Matteo Vicini, Claudio Conci, Giulio Cerullo, Roberto Osellame, Giuseppe Chirico, Emanuela Jacchetti, Manuela Teresa Raimondi
Published in: Materials Today Bio, Issue 35, 2025, Page(s) 102328, ISSN 2590-0064
Publisher: Elsevier BV
DOI: 10.1016/j.mtbio.2025.102328

High dioptric power micro-lenses fabricated by two-photon polymerization

Author(s): Giuseppe Chirico, Behjat Kariman, Alessandra Nardini, Mario Marini, Pablo Roldan, claudio Conci, Manuela Raimondi, Roberto Osellame, Giulio Cerullo, REBECA MARTINEZ VAZQUEZ
Published in: Optics Express, Issue Vol. 32, Issue 27, 2024, Page(s) 48114-48131, ISSN 1094-4087
Publisher: Optical Society of America
DOI: 10.1364/opticaopen.27231798.v1

A 3D millifluidic model of a dermal perivascular microenvironment on a chip

Author(s): Chiara Martinelli, Alberto Bocconi, Sofia Milone, Teresa Baldissera, Leonardo Cherubin, Giovanni Buccioli, Simone Perottoni, Claudio Conci, Giulio Cerullo, Roberto Osellame, Giuseppe Chirico, Emanuela Jacchetti, Manuela Teresa Raimondi
Published in: Lab on a Chip, Issue 25, 2025, Page(s) 423-439, ISSN 1473-0197
Publisher: Royal Society of Chemistry
DOI: 10.1039/d4lc00898g

Microlenses Fabricated by Two-Photon Laser Polymerization for Cell Imaging with Non-Linear Excitation Microscopy.

Author(s): Marini, M., Nardini, A., Vázquez, R. M., Conci, C., Bouzin, M., Collini, M., Osellame, R., Cerullo, G., Kariman, B. S., Farsari, M., Kabouraki, E., Raimondi, M. T., & Chirico, G.
Published in: Adv. Funct. Mater., 2023, Page(s) 2213926, ISSN 1616-301X
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/adfm.202213926

High-resolution lightweight and multifunctional 3D printed scaffolds for cell studies.

Author(s): Flamourakis, G., Kordas, A., Papageorgiou, M., Pateraki, V., Farsari, M., & Ranella, A.
Published in: Results in Materials, 2023, ISSN 2590-048X
Publisher: Elsevier
DOI: 10.1016/j.rinma.2023.100393

Agent-based modeling of macrophage-fibroblast interactions in the immune response to biomaterials

Author(s): Jennifer Riccio, Luca Presotto, Shir Bahiri, Liad Doniza, Donato Inverso, Laura Sironi, Uri Nevo, Giuseppe Chirico
Published in: PLOS One, Issue 20, 2025, Page(s) e0329186, ISSN 1932-6203
Publisher: Public Library of Science
DOI: 10.1371/journal.pone.0329186

Two-Photon Polymerization Lithography for Optics and Photonics: Fundamentals, Materials, Technologies, and Applications

Author(s): Wang, H., Zhang, W., Ladika, D., Yu, H., Gailevičius, D., Wang, H., Pan, C. F., Nair, P. N. S., Ke, Y., Mori, T., Chan, J. Y. E., Ruan, Q., Farsari, M., Malinauskas, M., Juodkazis, S., Gu, M., & Yang, J. K. W.
Published in: Advanced Functional Materials, 2023, ISSN 1616-301X
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/adfm.202214211