Skip to main content
Go to the home page of the European Commission (opens in new window)
English English
CORDIS - EU research results
CORDIS

AN IN VIVO BIOENGINEERED CHIP AS A SMART INTRAVITAL MULTIPHOTON IMAGING WINDOW FOR NEW VALIDATION PROTOCOLS OF BIOMATERIALS

Project description

Innovative microchip for in vivo analysis of biomaterials

The EU-funded IN2SIGHT project aims to introduce in vivo optical imaging for the innovative biocompatibility testing of biomaterials. The approach is based on a micro-structured chip for laboratory animal tissue implantation, containing features for spontaneous regeneration of vascularised tissue and a micro-lens array for in situ multi-spot imaging. This technology will dramatically improve light penetration in tissues, reducing image aberration issues and allowing quantitative and longitudinal analyses of the host inflammatory response to the implant. The project has the potential to enable quantification of the immune reaction to biomaterials at the cellular level, with a simultaneous reduction in the numbers of laboratory animals and the cost of biomaterial discovery in general.

Objective

Routine clinical use of biomaterials requires the reduction of the economical and ethical costs of biocompatibility tests (ISO10993 EU norm) which are unsustainable for small-medium industries and for the society. In this project we foster an unprecedented breakthrough in in-vivo optical imaging that will radically renew the biocompatibility tests of biomaterials.
A micro-structured chip, built by two-photon laser polymerization (2PP), will be implanted in lab animals, host a biomaterial and contain micro-features that guide the spontaneous regeneration of vascularized tissue within a thin gap (0.15mm) in contact with the biomaterial and act as beacons to correct the optical aberrations. The same chip carries a micro-lenses array for in-situ multi-spot imaging, with no need of external high numerical aperture objectives, dramatically improving light penetration in tissue. This chip will recast our thinking of deep tissue in-vivo imaging: the mice carry their own imaging optics, thus reducing substantially image aberration issues allowing unprecedented quantitative and longitudinal analyses of the host inflammatory response to the implant, without sacrificing the mice at each time step.
The project will allow unique quantification of the immune reaction to biomaterials at the cellular level (scientific impact), reduce (at least threefold) the number of used animals (societal impact) and the costs of biomaterial discovery (economical impact), and will Refine and Reduce protocols for biocompatibility on a single revolutionary device (regulatory impact). We open here a new visionary path for in-vivo imaging with high Replacement potential in oncological pharmaceutics and immune-therapies. 4 academic units, 1 public research institute and 2 SMEs ensure a highly inter-sectorial/interdisciplinary approach encompassing non-linear intravital imaging, bioengineering design, 2PP material science, biocompatibility protocols design and numerical simulations of immune response.

Fields of science (EuroSciVoc)

CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: https://op.europa.eu/en/web/eu-vocabularies/euroscivoc.

You need to log in or register to use this function

Keywords

Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)

Programme(s)

Multi-annual funding programmes that define the EU’s priorities for research and innovation.

Topic(s)

Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.

Funding Scheme

Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.

RIA - Research and Innovation action

See all projects funded under this funding scheme

Call for proposal

Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.

(opens in new window) H2020-FETOPEN-2018-2020

See all projects funded under this call

Coordinator

UNIVERSITA' DEGLI STUDI DI MILANO-BICOCCA
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 1 024 122,00
Address
PIAZZA DELL'ATENEO NUOVO 1
20126 Milano
Italy

See on map

Region
Nord-Ovest Lombardia Milano
Activity type
Higher or Secondary Education Establishments
Links
Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

€ 1 024 122,00

Participants (7)

My booklet 0 0