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AN IN VIVO BIOENGINEERED CHIP AS A SMART INTRAVITAL MULTIPHOTON IMAGING WINDOW FOR NEW VALIDATION PROTOCOLS OF BIOMATERIALS

Objective

Routine clinical use of biomaterials requires the reduction of the economical and ethical costs of biocompatibility tests (ISO10993 EU norm) which are unsustainable for small-medium industries and for the society. In this project we foster an unprecedented breakthrough in in-vivo optical imaging that will radically renew the biocompatibility tests of biomaterials.
A micro-structured chip, built by two-photon laser polymerization (2PP), will be implanted in lab animals, host a biomaterial and contain micro-features that guide the spontaneous regeneration of vascularized tissue within a thin gap (0.15mm) in contact with the biomaterial and act as beacons to correct the optical aberrations. The same chip carries a micro-lenses array for in-situ multi-spot imaging, with no need of external high numerical aperture objectives, dramatically improving light penetration in tissue. This chip will recast our thinking of deep tissue in-vivo imaging: the mice carry their own imaging optics, thus reducing substantially image aberration issues allowing unprecedented quantitative and longitudinal analyses of the host inflammatory response to the implant, without sacrificing the mice at each time step.
The project will allow unique quantification of the immune reaction to biomaterials at the cellular level (scientific impact), reduce (at least threefold) the number of used animals (societal impact) and the costs of biomaterial discovery (economical impact), and will Refine and Reduce protocols for biocompatibility on a single revolutionary device (regulatory impact). We open here a new visionary path for in-vivo imaging with high Replacement potential in oncological pharmaceutics and immune-therapies. 3 academic units, 2 public research institute and 2 SMEs ensure a highly inter-sectorial/interdisciplinary approach encompassing non-linear intravital imaging, bioengineering design, 2PP material science, biocompatibility protocols design and numerical simulations of immune response.

Call for proposal

H2020-FETOPEN-2018-2019-2020-01
See other projects for this call

Funding Scheme

RIA - Research and Innovation action

Coordinator

UNIVERSITA' DEGLI STUDI DI MILANO-BICOCCA
Address
Piazza Dell'ateneo Nuovo 1
20126 Milano
Italy
Activity type
Higher or Secondary Education Establishments
EU contribution
€ 994 122

Participants (6)

POLITECNICO DI MILANO
Italy
EU contribution
€ 685 435
Address
Piazza Leonardo Da Vinci 32
20133 Milano
Activity type
Higher or Secondary Education Establishments
IDRYMA TECHNOLOGIAS KAI EREVNAS
Greece
EU contribution
€ 503 125
Address
N Plastira Str 100
70013 Irakleio
Activity type
Research Organisations
TEL AVIV UNIVERSITY
Israel
EU contribution
€ 527 000
Address
Ramat Aviv
69978 Tel Aviv
Activity type
Higher or Secondary Education Establishments
DEUTSCHES KREBSFORSCHUNGSZENTRUM HEIDELBERG
Germany
EU contribution
€ 301 375
Address
Im Neuenheimer Feld 280
69120 Heidelberg
Activity type
Research Organisations
ASPHALION SL
Spain
EU contribution
€ 295 000
Address
Calle Tarragona, Numeros 151-157, Planta 10, Bloqu
08014 Barcelona
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
NANOTYPOS OE
Greece
EU contribution
€ 132 902
Address
Vepe Technopoli Thessaloniki Building C2
55535 Pylea Thessaloniki
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)