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OrganVision: Technology for real-time visualizing and modelling of fundamental process in living organoids towards new insights into organ-specific health, disease, and recovery

Project description

DEENESFRITPL

Transforming organoid research

Organoids are minuscule, three-dimensional tissue cultures from stem cells. They play an important role in personalised medicine and drug discovery. However, a tool that can help visualise organoids in real-time has not been realised. Addressing this, the EU-funded OrganVision project aims to create a real-time, high-resolution and label-free image processing technology for organoid research as well for the exploitation of new opportunities in this field. The project will show how each individual cell works in real-time on an engineered heart muscle, uncovering how stress and other factors can affect them.

Objective

OrganVision is a revolutionary technology proposition that will break new grounds in microscopy and develop an ideal imaging solution for organoid research. It will enable life scientists to visualize life unfolding in real-time inside the cells and tissue in an organ-mimicking living tissue environment (called organoid here). It will alter the paradigm in microscopy by converting the central obstacle of light scattering by thick samples into the central opportunity that enables 3D label-free imaging on organoids in real-time with sub-cellular (~200 nm) and inter-cellular resolution (~1 um) at speeds of >1 volume per second (cube of 100 um). For achieving this unprecedented feat, OrganVision will develop a new multi-physics solver that solves transport of intensity (ToI) and full wave electromagnetic (FWEM) models in a coupled manner. ToI provides 3D image with inter-cellular resolution and generates intensity distribution inside the sample. FWEM uses this intensity distribution to decode the near-field light interaction between the sub-cellular entities for generating 3D sub-cellular image. A novel microscope instrument delivers custom designed 3D illumination patterns at the speed of 200 patterns per second in order to solve the problem of ill-posedness encountered by these solvers. In order to exploit the opportunity thus created, OrganVision will develop an a computational model that models the dynamics and interactions of functional entities recorded by OrganVision imaging solution in order to identify the underlying mechanisms. The proof-of-concept will be shown on engineered heart tissue for real-time imaging of cell and tissue activity towards studying injury, repair and regeneration in heart muscle. OrganVision will transform microscopy from a visualization device to a knowledge discovery tool that will change the course of organoid research forever. We believe that OrganVision will lead to better understanding and faster therapy for several diseases.

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Call for proposal

H2020-FETOPEN-2018-2020

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Sub call

H2020-FETOPEN-2018-2019-2020-01

Funding Scheme

RIA - Research and Innovation action

Coordinator

UNIVERSITETET I TROMSOE - NORGES ARKTISKE UNIVERSITET
Net EU contribution
€ 973 991,25
Address
Hansine hansens veg 14
9019 Tromso
Norway

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Region
Norge Nord-Norge Troms og Finnmark
Activity type
Higher or Secondary Education Establishments
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Other funding
€ 0,00

Participants (7)

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EC signature date 27 January 2021
Last update: 23 July 2023

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Permalink: https://cordis.europa.eu/project/id/964800

European Union, 2023