Objective - To develop a novel vaccine candidate through isolation of appropriate antigens and cloning these into the pseudomonas lipoprotein vector;- To verify the efficiency of these vaccines in mice and dog models, the latter to be developed in Algeria and Morocco.Expected Outcome- Improved knowledge of the genetic basis of antigens of L. infantum and L. major;- Several antigens isolated, cloned on pseudomonas and tested in different animal models with regard to their vaccination efficiency;- Novel approach to develop and test a vaccine that might be useful also for other vaccine preparations. Results - Production of L. major lipoprotein/GP63 fusion protein.- Cloning of L. infantum GP63 gene.- Use of GP63 fusion protein to detect antibodies in human infection by L. major and L. infantum.- Use of GP63 fusion protein to elicit antibodies in mice and detection of GP63 variants in old and new world Leishmania.- Establishment of canine models for L. infantum and L. major infection.- Characterisation of GP63 from field isolates and cloning and subcloning of regions of interest of GP63 (protein analysis, gene expression, gene structures);- Cloning of the GP63 genes into the Pseudomonas vector;- Acquisition of basic data on specific immunological responses of the human and canine populations at risk;- Testing of cloned GP63 with regard to their immunogenic activity in dog and mouse models followed by immunising dog-models with the most efficient clones;- Development of a candidate vaccine based on the identified immunising clones;- Testing of resistance of immunised animals against natural parasites less or more virulent (L. major / mouse model) or recent field isolates (L. infantum / dog model);- Development of a diagnosis technology based on antigens and to be carried out on blood samples. Programme(s) IC-AVICENNE - Avicenne Initiative (Science and technology cooperation with the Maghreb and the countries of the Mediterranean Basin), 1991- Topic(s) Data not available Call for proposal Data not available Funding Scheme CSC - Cost-sharing contracts Coordinator Vrije Universiteit Brussel EU contribution No data Address 2,Pleinlaan 1050 Bruxelles Belgium See on map Total cost No data Participants (3) Sort alphabetically Sort by EU Contribution Expand all Collapse all Centre Hospitalo-Universitaire - Hopital Parnet Algeria EU contribution No data Address Husseyn-dey 16040 Alger See on map Total cost No data Institut Agronomique et Vétérinaire Hassan II Morocco EU contribution No data Address 6202 Rabat See on map Total cost No data UNIVERSIDAD DE MURCIA Spain EU contribution No data Address Avda. Teniente Flamesta 5 30003 MURCIA See on map Total cost No data
