Obiettivo - To develop a novel vaccine candidate through isolation of appropriate antigens and cloning these into the pseudomonas lipoprotein vector;- To verify the efficiency of these vaccines in mice and dog models, the latter to be developed in Algeria and Morocco.Expected Outcome- Improved knowledge of the genetic basis of antigens of L. infantum and L. major;- Several antigens isolated, cloned on pseudomonas and tested in different animal models with regard to their vaccination efficiency;- Novel approach to develop and test a vaccine that might be useful also for other vaccine preparations. Results - Production of L. major lipoprotein/GP63 fusion protein.- Cloning of L. infantum GP63 gene.- Use of GP63 fusion protein to detect antibodies in human infection by L. major and L. infantum.- Use of GP63 fusion protein to elicit antibodies in mice and detection of GP63 variants in old and new world Leishmania.- Establishment of canine models for L. infantum and L. major infection.- Characterisation of GP63 from field isolates and cloning and subcloning of regions of interest of GP63 (protein analysis, gene expression, gene structures);- Cloning of the GP63 genes into the Pseudomonas vector;- Acquisition of basic data on specific immunological responses of the human and canine populations at risk;- Testing of cloned GP63 with regard to their immunogenic activity in dog and mouse models followed by immunising dog-models with the most efficient clones;- Development of a candidate vaccine based on the identified immunising clones;- Testing of resistance of immunised animals against natural parasites less or more virulent (L. major / mouse model) or recent field isolates (L. infantum / dog model);- Development of a diagnosis technology based on antigens and to be carried out on blood samples. Programma(i) IC-AVICENNE - Avicenne Initiative (Science and technology cooperation with the Maghreb and the countries of the Mediterranean Basin), 1991- Argomento(i) Data not available Invito a presentare proposte Data not available Meccanismo di finanziamento CSC - Cost-sharing contracts Coordinatore Vrije Universiteit Brussel Contributo UE Nessun dato Indirizzo 2,Pleinlaan 1050 Bruxelles Belgio Mostra sulla mappa Costo totale Nessun dato Partecipanti (3) Classifica in ordine alfabetico Classifica per Contributo UE Espandi tutto Riduci tutto Centre Hospitalo-Universitaire - Hopital Parnet Algeria Contributo UE Nessun dato Indirizzo Husseyn-dey 16040 Alger Mostra sulla mappa Costo totale Nessun dato Institut Agronomique et Vétérinaire Hassan II Marocco Contributo UE Nessun dato Indirizzo 6202 Rabat Mostra sulla mappa Costo totale Nessun dato UNIVERSIDAD DE MURCIA Spagna Contributo UE Nessun dato Indirizzo Avda. Teniente Flamesta 5 30003 MURCIA Mostra sulla mappa Costo totale Nessun dato
