Objective
OBTENTION OF NEW VACCINES AGAINST HERPESVIRUSES AFFECTING PORCINE AND BOVINE (AUJESKI'DISEASE AND INFECTIOUS BOVINE RHINOTRACHEITIS).
The antibody response of pigs to glycoprotein I (gI) of pseudorabies virus was used to distinguish between infected and vaccinated pigs. We identified 6 epitopes on gI of which 5 were localised. The amino acid sequence of the 3 continuous epitopes was determined. The 2 discontinuous epitopes were more immunogenic in pigs than the 3 continuous ones. Amino acids were deleted in these epitopes by site directed mutagenesis using the polymerase chain reaction.
Vaccinia virus was used as a vector for glycoproteins gI, gIV and gH of bovine herpesvirus type 1 (BHV-1). Cattle vaccinated with these recombinant viruses were not protected against challenge with virulent BHV-1. Thus, these glycoproteins expressed by a heterologous viral vector provided insufficient immunity. Five distinct antigenic sites were found on gII of pseudorabies virus (PRV), 3 of which were located close to the transmembrane region. The gII gene was inserted into vaccinia virus and this recombinant virus induced neutralising antibodies in pigs. Six different epitopes were localised on gI of PRV, of which 3 were discontinuous. The amino acid sequences of the 3 continuous epitopes were determined. The discontinuous epitopes appeared to be the most immunogenic in pigs. Tests to detect antiboides to gI must include the discontinuous epitopes.
AT THE PRESENT STAGE OF HERPESVIRUS RESEARCH THERE IS HOPE THAT SUBUNIT VACCINES CONTAINING HERPESVIRAL GLYCOPROTEINS (GP), OR RECOMBINANT VACCINES EXPRESSING HERPESVIRAL GLYCOPROTEIN GENES WILL REPRESENT SAFE AND MORE EFFICIENT VACCINES. PRV AND BHV-1 PHYLOGENETICALLY CLOSELY RELATED VIRUSES AND PARTS OF THE GENOMES CROSS-HYBRIDIZE. THE EXPERIMENTAL STRATEGIES ARE CONVERGENT AND THEREFORE THE THREE GROUPS BELIEVE THAT CO-OPERATION WILL SPEED UP RESEARCH.
THE PROJECT FOCUSSES ON THE:
1.- IDENTIFICATION OF GP'S
2.- ROLE OF INDIVIDUAL GP'S IN VIRULENCE AND IMMUNOGENICITY
3.- LOCALIZATION OF THEIR GENES TO BE FOLLOWED BY
4.- ISOLATION OF GENES AND
5.- INSERTION OF GP GENES IN APPROPRIATE RECOMBINANT VECTORS OR EXPRESSIONS SYSTEMS.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences microbiology virology
- medical and health sciences basic medicine pharmacology and pharmacy pharmaceutical drugs vaccines
- agricultural sciences animal and dairy science domestic animals animal husbandry
- natural sciences chemical sciences organic chemistry amines
- natural sciences biological sciences genetics genomes
You need to log in or register to use this function
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Data not available
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Data not available
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
8200 AJ LELYSTAD
Netherlands
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.