Objetivo Studies were aimed at identifying, characterizing and defining the roles of the gene products of poraine transmissable gastroenteritis virus (TGEV) in pathogenesis and immunity by means of recombinant deoxyribonucleic acid (DNA) technology. Two of the viral strucutral proteins were glycosylated and one of them, the peplomer, contained regions to which antiboides could attach, thereby preventing viral growth. In order to express the peplomer protein and identify regions involved in immunity, the isolation, sequencing and expression of TGEV genes in recombinant viruses and in yeast was carried out. Seven TGEV genes were identified and sequenced. Three of the gene products, nucleoprotein, integral membrane protein and peplomer, were involved in the structure of the virus particle and have been expressed in bacteria, yeast and vaccinia virus. Specific antibodies were raised against the structural proteins and these led to the identification of a specific antibody binding site on the nucleoprotein and several specific sites on the peplomer. The peplomer protein expressed by vaccinia virus still reacted with all the specific antibodies and the relative positions of 4 antigenic sites were identified on the peplomer protein. Antisera to the peplomer expressed by vaccinia appeared to prevent viral growth and disease in piglets. Ámbito científico ciencias naturalesciencias biológicasmicrobiologíabacteriologíaciencias naturalesciencias biológicasmicrobiologíavirologíaciencias naturalesciencias biológicasgenéticaADNciencias naturalesciencias biológicasbioquímicabiomoléculasproteínas Programa(s) FP1-BAP - Multiannual research action programme (EEC) in the field of biotechnology (BAP), 1985-1989 Tema(s) Data not available Convocatoria de propuestas Data not available Régimen de financiación CSC - Cost-sharing contracts Coordinador CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS Dirección Serrano 117 Madrid España Ver en el mapa Aportación de la UE Sin datos
