Objective A reverse phase high performance liquid chromatography (HPLC) method to separate adenosine triphosphate (ATP), adenosine diphosphate (ADP), adenosine monophosphate (AMP), inosine monophosphate (IMP), inosine and adenosine, with a detection limit of 3 to 5 pmol, was developed. Using this method to identify the extracellular ATP degradation products at the innervated frog sartorius muscle, the following ectoenzymes were characterised: ATPase, ADPase, 5'-nucleotidase, AMP deaminase. 2 alternative pathways for the degradation of extracellular AMP were also identified along with inhibitors of extracellular AMP metabolism. Fields of science natural sciencesbiological sciencesgeneticsnucleotides Programme(s) FP1-BAP - Multiannual research action programme (EEC) in the field of biotechnology (BAP), 1985-1989 Topic(s) Data not available Call for proposal Data not available Funding Scheme CSC - Cost-sharing contracts Coordinator FUNDACAO CALOUSTE GULBENKIAN Address Ciencia apartado 14 2781 Oeiras Portugal See on map EU contribution € 0,00
