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IN VITRO EVALUATION OF THE PHARMACOLOGICAL ACTIVITY OF ANALOGUES OF ADENINE NUCLEOTIDES AND ADENOSINE : STRUCTURE-ACTIVITY REQUIREMENTS OF ECTONUCLEOTIDASES

Objective


A reverse phase high performance liquid chromatography (HPLC) method to separate adenosine triphosphate (ATP), adenosine diphosphate (ADP), adenosine monophosphate (AMP), inosine monophosphate (IMP), inosine and adenosine, with a detection limit of 3 to 5 pmol, was developed. Using this method to identify the extracellular ATP degradation products at the innervated frog sartorius muscle, the following ectoenzymes were characterised: ATPase, ADPase, 5'-nucleotidase, AMP deaminase. 2 alternative pathways for the degradation of extracellular AMP were also identified along with inhibitors of extracellular AMP metabolism.

Funding Scheme

CSC - Cost-sharing contracts

Coordinator

FUNDACAO CALOUSTE GULBENKIAN
Address
Ciencia Apartado 14
2781 Oeiras
Portugal