Objetivo A reverse phase high performance liquid chromatography (HPLC) method to separate adenosine triphosphate (ATP), adenosine diphosphate (ADP), adenosine monophosphate (AMP), inosine monophosphate (IMP), inosine and adenosine, with a detection limit of 3 to 5 pmol, was developed. Using this method to identify the extracellular ATP degradation products at the innervated frog sartorius muscle, the following ectoenzymes were characterised: ATPase, ADPase, 5'-nucleotidase, AMP deaminase. 2 alternative pathways for the degradation of extracellular AMP were also identified along with inhibitors of extracellular AMP metabolism. Ámbito científico natural sciencesbiological sciencesgeneticsnucleotides Programa(s) FP1-BAP - Multiannual research action programme (EEC) in the field of biotechnology (BAP), 1985-1989 Tema(s) Data not available Convocatoria de propuestas Data not available Régimen de financiación CSC - Cost-sharing contracts Coordinador FUNDACAO CALOUSTE GULBENKIAN Aportación de la UE Sin datos Dirección CIENCIA APARTADO 14 2781 OEIRAS Portugal Ver en el mapa Coste total Sin datos
