Objective
Many products of major therapeutic value can be obtained only from animal cells grown in culture. The aim of this project is to improve the processes currently employed in this sector of biotechnology with special emphasis on the vectors and promoters used and on the quality of post translational modification.
It is anticipated that in the future, monoclonal antibodies will be routinely isolated from phage libraries. Similarly, the expression of binding domains derived from antibodies within different cellular compartments of mammalian cells is likely to become a widely used technique to inhibit the function of recognised molecules. An important feature of the phage antibody technology is that the process of antibody selection is simultaneous with that of the cloning of the corresponding encoding sequences.
It would, therefore, be highly desirable that vectors for the expression of antibody domains within mammalian cells should be compatible with the phage vectors used to select antibody specificities. The development of such an integrated vector system for the expression of immunoglobulin domains within different cellular compartments of mammalian cells after they have been selected from phage antibody libraries is the objective of this project.
By the end of this project we would expect a researcher to be able to screen a phage library of diversity large enough to allow the selection of a monoclonal phage antibody (as ScFv of FAb) which recognises the antigen used for screening at a useful affinity. The cloning of the antibody binding specificity into an appropriate vector using convenient and consistent sites would allow expression of the selected antibody in the most suitable form (as ScFv, FAb, or complete antibody) within the particular cellular compartment of interest of a mammalian cell (intracytoplasmic, nuclear, secretory, endoplasmic reticulum), by the incorporation of appropriate protein tags at N or C terminal ends. The use of different promoters (tissue specific, inducible, constitutive) and vectors (plasmid, retroviral) will allow further refinement of expression. By this means the expression of targeted molecules is expected to be inhibited in situ.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences microbiology virology
- natural sciences biological sciences biochemistry biomolecules proteins
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
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Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
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Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
00198 Roma
Italy
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.