Cooperative research on late somatic effects on ionazing radiation in the mammalian organism

Información del proyecto

EULEP

Identificador del acuerdo de subvención: CIPD930418

Proyecto cerrado

Fecha de inicio 1 Abril 1994

Fecha de finalización 31 Mayo 1996

Financiado con arreglo a

• Scientific and technological cooperation between the European Community and European non-member countries, 1992-

Coste total

Sin datos

Aportación de la UE

Sin datos

Coordinado por

EUROPEAN LATE EFFECTS PROJECT GROUP
Belgium

Objetivo

IN THE PROPOSED EXPERIMENTS WE WISH TO IRRADIATE MOUSE FETUS IN UTERO WITH VARIOUS DOSES OF C060 GAMMA RADIATION AND EXAMINE BOTH THE DOSE DEPENDENT Tumor INDUCTION AND THE ACTIVATION 0F ONCOGENES (ras FAMILY, myc) AND INACTIVATION 0F TUMOR SUPPRESSOR GENES (P53, RETINOBLASTOMA PROTEIN) IN THE DEVELOPED TUMORS. DETAILS :
1. MOUSE FETUS WILL BE IRRADIATED IN UTERO WITH DIFFERENT DOSES 0F C060 GAMMA IRRADIATION AT DIFFERENT DAYS OF GESTATION (TABLE 1). TO MEASURE THE TUMOR INDUCTION MICE WILL BE SACRIFICED WHEN THEY LOOK VISIBLE ILL 0R AT TWO YEARS OF AGE. THE Tumor INCIDENCE 0F THE IRRADIATED AND THE UNIRRADIATED CONTROL MICE WILL BE COMPARED.
2. THE EXPRESSION OF THE K-, H-, N-ras AND myc ONCOGENES, AS WELL AS THE p53 AND Rb TUMOR SUPPRESSOR GENES WILL BE EXAMINED BOTH IN THE RADIATION INDUCED AND SPONTANEOUSLY DEVELOPED TUMORS.
3. THE AMPLIFICATION AND REARRANGEMENT oF THE ABOVE MENTIONED ONCOGENES, TUMOR SUPPRESSOR GENES WILL BE INVESTIGATED, TOO. 4. WE DETERMINE WHETHER THE ACTIVATION oF THE ras ONCOGENE FAMILY (K-, H- AND N-ras) AND THE INACTIVATION/ONCOGENIC ACTIVATION OF THE p53 PROTEIN ARE THE RESULTS OF POINT MUTATIONS IN THE CODING REGION OF THESE GENES. THE POSITION 0F THE MUTATIONS WILL BE DETERMINED BOTH IN RADIATION INDUCED AND SPONTANEOUS TUMORS.
5. COMPARING THE MENTIONED GENETIC CHANGES IN TUMORS OF IRRADIATED AND UNIRRADIATED MICE, WE INVESTIGATE WHETHER IDENTICAL ALTERATIONS OCCUR DURING THE RADIATION INDUCED AND SPONTANEOUS CARCINOGENESIS. WE ALSO EXAMINE WHETHER IDENTICAL CHANGES DEVELOP IN DIFFERENT TUM0R TYPES.

Ámbito científico (EuroSciVoc)

Este proyecto aún no se ha clasificado con EuroSciVoc.
Sugiera los ámbitos científicos que considere más relevantes y ayúdenos a mejorar nuestro servicio de clasificación.

Programa(s)

• IC-PECO/COPERNICUS - Scientific and technological cooperation between the European Community and European non-member countries, 1992-

Tema(s)

• 0501 - CEEC participation in NUCLEAR FISSION SAFETY programme (RADIATION PROTECTION)

Convocatoria de propuestas

Datos no disponibles

Régimen de financiación

CSC - Cost-sharing contracts

Coordinador

EUROPEAN LATE EFFECTS PROJECT GROUP

• Aportación de la UE: Sin datos
• Dirección: RUE CHARLES LEMAIRE,1 ; 1160 BRUSSELS ; Bélgica
• Coste total: Sin datos

Participantes (1)

"FREDERIC JOLIOT CURIE" NATIONAL RESEARCH INSTITUTE FOR RADIOBIOLOGY AND RADIOHYGIENE - NATIONAL CENTER OF PUBLIC HEALTH

Hungría

• Aportación de la UE: Sin datos
• Dirección: ANNA U.5 ; 1775 BUDAPEST
• Coste total: Sin datos