Monitoring of polychlorinated biphenyls (PCBs) in ground waters through an immunochemical biosensor with electrochemical detection

Objective

To develop immunochemical biosensors with electrochemical detection based on enzyme-antibodies and enzyme-PCB conjugates with glucose oxidase (GOD), alkaline phosphatase (AP) and/or peroxidase (P) enzymes;

to develop disposable strips for enzyme activity measurement;

to develop a flow injection system (FIA) for continuous monitoring of PCBs in water supply;

to develop a portable instrument for amperometric measurement with disposable strips;

to compare and assess the two prototypes with existing procedures for PCB determination.


The programme can be divided in 11 different tasks:

1. Production of Electrochemical Biosensor for enzyme activity determination.

2. Production of immobilized antigens or antibodies.

3. Development of electrochemical biosensors for PCB with immobilized antigens.

4. Validation of PCB biosensors in comparison to establish reference procedures.

5. Development of a continuous monitoring procedure.

6. Construction of a prototype for continuous monitoring of PCBs in field.

7. Validation of the continuous monitoring with biosensors using established reference procedures.

8. Development of disposable strips with immobilized PCBs.

9. Development of a procedure for measuring PCBs with disposable strips.

10. Development of a prototype for PCB analysis with disposable strips.

11. Validation of disposable strip measurements using established reference procedures.

Fields of science

• engineering and technology › electrical engineering, electronic engineering, information engineering › electronic engineering › sensors › biosensors
• natural sciences › chemical sciences › electrochemistry
• natural sciences › biological sciences › biochemistry › biomolecules › proteins › enzymes

Programme(s)

• FP3-ENV 1C - Specific research and technological development programme (EEC) in the field of the environment, 1990-1994

Topic(s)

• 0201 - Assessment of environmental quality and monitoring

Call for proposal

Data not available

Funding Scheme

CSC - Cost-sharing contracts

Coordinator

UNIVERSITY OF FLORENCE

Address

Viale Morgagni 48
50134 Firenze
Italy

Participants (3)

Ente Nazionale per l'Energia Elettrica SpA (ENEL)

Italy

Address

Via Dalmazia 21/c
72100 Brindisi

Institut für Chemo- und Biosensorik e.V.

Germany

Address

Mendelstrasse 7
48149 Münster

Universidad Autonoma de Barcelona

Spain

Address

08193 Barcelona