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Degradation of xenobiotics : an investigation of microbiological events occuring within polluted sites with emphasis on those undergoing bioremediation

Objective

The main objective of the project is the determination of the relative competitiveness and genetic stability of representative soil-derived xenobiotic degrading bacteria of different genera (eg. Gram-negative vs Gram-positive) using different catabolic pathways for the same or related aromatic xenobiotics.

Competition experiments will be designed to mimic some of the conditions observed in both polluted sites and in situ biorestoration treatments, ie. multi-strain/multi-carbon source systems. At the same time genetic and biochemical studies will be initiated on strains being used in the competition studies, in particular the Gram-positive strains which represent a poorly characterised class.


The experimental work will be divided into two parts :

- Competition experiments between a selected number of representative strains of different biochemical and taxonomic groups of bacteria under different environmental conditions in both chemostat cultures and soil microcosms.

- Characterisation of xenobiotic-degrading strains by genetic and biochemical analysis of their catabolic pathways.

Funding Scheme

CSC - Cost-sharing contracts

Coordinator

UNIVERSITY OF WALES - BANGOR
Address
Deiniol Road
LL57 2UW Bangor - Gwynedd
United Kingdom

Participants (2)

CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS
Spain
Address
1,Profesor Albareda 1
18008 Granada
NATIONAL INSTITUTE OF PUBLIC HEALTH AND ENVIRONMENT
Netherlands
Address
9,Antonie Van Leeuwenhoeklaan 9
3720 BA Bilthoven