Objective The project is concerned with the discovery and design of stable peptides as biding ligands for antibodies and to produce stable affinity media for the efficient purificationof recombinant antibody produced in plants.The intention is not to use whole or even fragmented antigen on the affinity surface but rather to use small stable peptides which mimic the natural epitope recognised by the antibody. The idealligand will allow controlled capture and release during processing thereby yielding purified active antibody.The project focuses on this extraction process because the effectiveness of downstream activities are crucial to the vision of low cost production of antibody fragments as fine chemicals produced by agricultural technology.The objectives of the project are :- to develop and scale-up antibody purification processes that exploit the antibody's inherent ability to bind its epitope, for maximum recovery of active material from plant tissues;- to devise specific affinity purification media, based on peptide antigen analogues designed to capture and release antibodies, in a stable and multiple re-useable system;- to transfect genes of appropriately designed antibodies into tobacco and potato (production organisms), to provide material on a practical scale for process development;- to develop process control and product QC techniques to quantify, monitor and validate the production and purification processes;- to integrate plant culture and harvesting with tissue extraction and affinity processing to develop and define a scale-up pilot process;- to evaluate the performance characteristics of the working pilot system in terms of active product yield, reusability/stability of immobilised peptide, cost efficiency and ease of further scale-up. Fields of science natural sciencesbiological sciencesbiochemistrybiomoleculesagricultural sciencesagriculture, forestry, and fisheriesagriculture Programme(s) FP4-FAIR - Specific research, technological development and demonstration programme in the field of agriculture and fisheries (including agro-industry, food technologies, forestry, aquaculture and rural development), 1994-1998 Topic(s) 2.2 - Bioprocessing Call for proposal Data not available Funding Scheme CSC - Cost-sharing contracts Coordinator Unilever UK Central Resources Ltd Address Colworth house MK44 1LQ Sharnbrook United Kingdom See on map EU contribution € 0,00 Participants (2) Sort alphabetically Sort by EU Contribution Expand all Collapse all I.D. - D.L.O. - INSTITUTE FOR ANIMAL SCIENCE AND HEALTH RESEARCH Netherlands EU contribution € 0,00 Address Edelhertweg, 15 - p.o. box 15 8200 AB Lelystad See on map TECHNISCHEN HOCHSCHULE AACHEN Germany EU contribution € 0,00 Address Worringerweg, 1 52074 Aachen See on map
