Objective
Fishery products comprise a wide variety of species and products from which is difficult to know the composition, especially in the case of thermally processed seafood products. Identification of fish species in these products, by specific protein profiles, could be hindered by the denaturation of these proteins caused by the severe thermal treatment used during the elaboration of these products. The use of DNA analysis as a tool for species identification has shown to be successful for identifying canned tuna species (AIR2 CT94-1126). The use of DNA diagnostic techniques for different groups of products could be of value since in some cases there is not a reliable method of identification, such in sterilised products.
In other cases the presence of several sources of fish can impede the identification. It is thought that DNA analysis techniques could solve several authentication problems up to now unresolved.
The work program in this project will involve the study of the following type of products:
a. Sterilised products
Canned fish and mollusc
Baby Food
Fish Pate
b. Heated Products
Smoked Fish
Fish meal
Pre-cooked Fish and mollusc
c. Non-heated Products
Caviar
This study will comprise several steps needed for the determination of the best methodology to be applied in each case:
1. Collection, authentication and preparation of products.
2. Developing of DNA isolation methods suited to each product.
3. Cytochrome b and Control region DNA sequencing in species of interest.
4. Designing of primers for the obtaining of DNA fragments from the products.
5. Development of secondary DNA- diagnostic methods based on analysis of PCR amplicons.
6. Validation of the diagnostic methods.
7. Creation of a database for fish species identification accessible via Internet.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences computer and information sciences internet
- natural sciences computer and information sciences databases
- natural sciences biological sciences genetics DNA
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences biological sciences zoology ichthyology
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Multi-annual funding programmes that define the EU’s priorities for research and innovation.
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Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
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Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
36208 VIGO
Spain
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.