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Contenido archivado el 2024-05-14

Fate, activity and threat of ralstonia solanacearum, the causal agent of potato brownrot, in european soils,rhizospheres and water systems

Objetivo

Potato brownrot, the disease caused by Ralstonia solanacearum, is a serious emerging problem in European agriculture, with severe losses recorded in recent years in potato cropping. Several recent reports have indicated that the causal agent, R. solanacearum race 3, may have established in European soil, plant and water systems in various countries of the Union. This proposal by 5 European expert laboratories aims to assess the severity of infestation by the pathogen, as well as the effects of simple non-chemical practical methods taken against it. The project will first fine-tune available methods for the detection, identification and ecological monitoring of the brownrot bacterium. Partners will subsequently perform a European transect study aimed at investigating the prevalence as well as load of R. solanacearum in several European ecosystems. This study will provide information as to pathogen incidence, as well as generate strains which will serve as target organisms for subsequent ecological assessments of fate and persistence. Such strains will also be characterized for their aggressiveness versus a broad selection of potential host plants. In a third phase, the experiments will focus on the fate of R. solanacearum strains selected in the transect study, following a release from diseased potato tubers in soil, its fate should it enter waterways and the potential for a diversity of plants to serve as reservoirs of the bacterium. The capability of the bacterium to enter a viable-nonculturable state, which would escape current certified detection methods will be assessed. Furthermore, the response of R. solanacearum to stressful conditions encountered in soil and water systems, potentially leading to a multiply-stress resistant physiological state, will be determined. The data on ecological fate obtained in soil will be linked to feasible practical measures, i.e. effects of solarization, inundation, covering of fields with farmer's plastic and addition of organic materials will be assessed. Data obtained in water systems will serve to predict R. solanacearum persistence and spread, its potential for growth at low substrate concentrations, and its extinction as a result of the prevailing ecological conditions. A fourth project task will address the potential for development of an antagonistic microflora in selected soil systems, resulting in suppressive soils. An advanced molecular fingerprinting method will be used hand-inhand with a cultivation-based approach, in order to unravel the structure of the microbial communities in suppressive versus conducive situations, and a link will be sought between both data sets. Dominant members of the culturable fraction will be screened for their capacity to inhibit R. solanacearum. The aggressiveness of selected novel isolates will be determined and used in an assessment of the threat these pose to European potato cropping. In addition, an R. solanacearum resistance gene with potential for use in potato will be tested for its capacity to ward off infection pressure by a range of pathogen isolates. The data obtained from the transect study, from the ecological fate studies and from the suppressiveness studies set in the perspective of farmer's measures, will serve to define a set of recommendations (guidelines) useful in the definition of feasible non-chemical disease management practices.

Ámbito científico (EuroSciVoc)

CORDIS clasifica los proyectos con EuroSciVoc, una taxonomía plurilingüe de ámbitos científicos, mediante un proceso semiautomático basado en técnicas de procesamiento del lenguaje natural. Véas: El vocabulario científico europeo..

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Coordinador

DLO RESEARCH INSTITUTE FOR PLANT PROTECTION
Aportación de la UE
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Dirección
Binnenhaven 5
6700 WAGENINGEN
Países Bajos

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Participantes (4)

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