Objective The first objective of the work will be the cDNA cloning of the genes encoding two autoantigens specific for the Golgi complex, p210 and p230, by immunological screening of two cDNA expression libraries. The sequence of these clones will be analysed in order to characterize the structure, to understand the function of the proteins and to know wether they are included in a new family of proteins associated to the Golgi apparatus that are preferent targets in certain autoimmune diseases. Fragments of the proteins will be subcloned into a bacterial expression vector in order to overproduce the peptides and to obtain antibodies against p210 and p230. Finally, clones encoding wild type or mutants p210 or p230 will be transfected into eucaryotic cells in order to study the domains involved in the localization and interactions of these proteins in the cell. Fields of science natural sciencesbiological sciencesbiochemistrybiomoleculesproteinsmedical and health sciencesbasic medicineimmunologyautoimmune diseasesnatural sciencesmathematicspure mathematicsmathematical analysisfunctional analysis Programme(s) FP4-TMR - Specific research and technological development programme in the field of the training and mobility of researchers, 1994-1998 Topic(s) 0302 - Post-doctoral research training grants TL05 - Cell Biology Call for proposal Data not available Funding Scheme RGI - Research grants (individual fellowships) Coordinator UNIVERSIDAD DE SEVILLA EU contribution No data Address S/N,Profesor Garcia Gonzalez S/N 41012 SEVILLA Spain See on map Total cost No data Participants (1) Sort alphabetically Sort by EU Contribution Expand all Collapse all Not available Spain EU contribution No data Address See on map Total cost No data