Cloning and sequencing of the cdna coding for two golgi autoantigens ; functional analysis by site-directed mutagenesis

Project Information

Grant agreement ID: FMBI950044

Project closed

Start date 1 May 1996

End date 30 April 1997

Funded under

• Specific research and technological development programme in the field of the training and mobility of researchers, 1994-1998

Total cost No data

EU contribution No data

Coordinated by

UNIVERSIDAD DE SEVILLA
Spain

Objective

The first objective of the work will be the cDNA cloning of the genes encoding two autoantigens specific for the Golgi complex, p210 and p230, by immunological screening of two cDNA expression libraries.
The sequence of these clones will be analysed in order to characterize the structure, to understand the function of the proteins and to know wether they are included in a new family of proteins associated to the Golgi apparatus that are preferent targets in certain autoimmune diseases. Fragments of the proteins will be subcloned into a bacterial expression vector in order to overproduce the peptides and to obtain antibodies against p210 and p230. Finally, clones encoding wild type or mutants p210 or p230 will be transfected into eucaryotic cells in order to study the domains involved in the localization and interactions of these proteins in the cell.

Fields of science

• natural sciences › biological sciences › biochemistry › biomolecules › proteins
• medical and health sciences › basic medicine › immunology › autoimmune diseases
• natural sciences › mathematics › pure mathematics › mathematical analysis › functional analysis

Programme(s)

• FP4-TMR - Specific research and technological development programme in the field of the training and mobility of researchers, 1994-1998

Topic(s)

• 0302 - Post-doctoral research training grants
• TL05 - Cell Biology

Call for proposal

Data not available

Funding Scheme

RGI - Research grants (individual fellowships)

Coordinator

UNIVERSIDAD DE SEVILLA

• EU contribution: No data
• Address: S/N,Profesor Garcia Gonzalez S/N ; 41012 SEVILLA ; Spain
• Total cost: No data

Participants (1)

Not available

Spain

• EU contribution: No data
• Total cost: No data