Obiettivo The first objective of the work will be the cDNA cloning of the genes encoding two autoantigens specific for the Golgi complex, p210 and p230, by immunological screening of two cDNA expression libraries. The sequence of these clones will be analysed in order to characterize the structure, to understand the function of the proteins and to know wether they are included in a new family of proteins associated to the Golgi apparatus that are preferent targets in certain autoimmune diseases. Fragments of the proteins will be subcloned into a bacterial expression vector in order to overproduce the peptides and to obtain antibodies against p210 and p230. Finally, clones encoding wild type or mutants p210 or p230 will be transfected into eucaryotic cells in order to study the domains involved in the localization and interactions of these proteins in the cell. Campo scientifico natural sciencesbiological sciencesbiochemistrybiomoleculesproteinsmedical and health sciencesbasic medicineimmunologyautoimmune diseasesnatural sciencesmathematicspure mathematicsmathematical analysisfunctional analysis Programma(i) FP4-TMR - Specific research and technological development programme in the field of the training and mobility of researchers, 1994-1998 Argomento(i) 0302 - Post-doctoral research training grants TL05 - Cell Biology Invito a presentare proposte Data not available Meccanismo di finanziamento RGI - Research grants (individual fellowships) Coordinatore UNIVERSIDAD DE SEVILLA Contributo UE Nessun dato Indirizzo S/N,Profesor Garcia Gonzalez S/N 41012 SEVILLA Spagna Mostra sulla mappa Costo totale Nessun dato Partecipanti (1) Classifica in ordine alfabetico Classifica per Contributo UE Espandi tutto Riduci tutto Not available Spagna Contributo UE Nessun dato Indirizzo Mostra sulla mappa Costo totale Nessun dato
