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Contenu archivé le 2024-06-10

Molecular mechanisms involved in the phagosome-endosome fusion

Objectif



Phagocytosis is the uptake by cells of large particles into vacuoles. During this process, newly formed phagosomes fuse with endosomes and lysosomes. The Griffiths group has established the use of latex beads with macrophages as a model for studying phagocytosis, and set up an in vitro fusion assay between phagosomes and endocytic organelles. Several data suggest a role of actin in the phagosome-endosome fusion. I propose to characterize membrane and actin interactions, by identifying membrane proteins of the phagosome which directly or indirectly bind actin. The major proteins will be identified using high resolution 2D gel electrophoresis, and a collaboration could be set up with groups having expertise in molecular biology. This line of research will allow to more understand some steps in phagocytosis which plays a central role during host defense against infective agents.

Champ scientifique (EuroSciVoc)

Programme(s)

Thème(s)

Appel à propositions

Données non disponibles

Régime de financement

RGI - Research grants (individual fellowships)

Coordinateur

European Molecular Biology Laboratory
Contribution de l’UE
Aucune donnée
Adresse
1,Meyerhofstraße
69117 Heidelberg
Allemagne
Coût total
Aucune donnée

Participants (1)