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Contenu archivé le 2024-04-30

Extension of the range of double-stranded dna sequences which can be recognized by oligonucleotides via triple helix formation

Objectif



The growing interest raised in olignucleotide-directed triple helix formations ('anti-gene strategy!) is due to their potential biological and therapeutical applications for controling gene expression at both transcriptional and replicational levels. The present project is aimed at developing new approaches to extend the range of double-stranded DNA recognition via oligonucleotide-directed triple helix formation. The ultimate goal is to apply 'antigene strategy' to any DNA target sequence with biological interest without sequence limitation as it is currently restricted to polypurine-polypyrimidine tracts. Syntheses of modified oligonucleotides containing triple helix-specific stabilizers are envisioned. Their abilities to form stable extended triple helices under physiological conditions will be evaluated. Results are expected in inhibition of gene expression in oncogenes and HIV viral genes.

Champ scientifique (EuroSciVoc)

Programme(s)

Thème(s)

Appel à propositions

Données non disponibles

Régime de financement

RGI - Research grants (individual fellowships)

Coordinateur

Muséum National d'Histoire Naturelle
Contribution de l’UE
Aucune donnée
Adresse
43,Rue Cuvier 43
75231 Paris
France
Coût total
Aucune donnée

Participants (1)