Objective Currently, there has been considerable interest in the mechanisms associated with radical-induced DNA cleavage. Apart from the established intermediacy of radicals in the C4' and C-5' positions of the sugar backbone, there has been substantial experimental evidence that C-l' radicals are also intermediates in the reaction between neocarzinostatin and double stranded DNA containing dAGC sequences and also in the reaction of bleomycin with oligonucleotides modified with a single ribo or arabino nucleoside. The goals of the proposed research will be to prepare a number of modified nucleosides and oligonucleotides, with modifications that can generate specifically a single C-1' radical species and study its degradation under a variety of condiiions. Moreover, we plan to design a second set of modifications which can specifically trap an in vitro generated C-1' radical species, this being either a carbon centered or oxygen radical. Fields of science natural sciencesbiological sciencesgeneticsDNA Programme(s) FP4-TMR - Specific research and technological development programme in the field of the training and mobility of researchers, 1994-1998 Topic(s) 0302 - Post-doctoral research training grants TC03 - Biological, Medicinal and Pharmaceutical Chemistry Call for proposal Data not available Funding Scheme RGI - Research grants (individual fellowships) Coordinator NATIONAL RESEARCH COUNCIL OF ITALY EU contribution No data Address Via piero gobetti 101 40129 Bologna Italy See on map Total cost No data Participants (1) Sort alphabetically Sort by EU Contribution Expand all Collapse all Not available Greece EU contribution No data Address See on map Total cost No data