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Zawartość zarchiwizowana w dniu 2024-04-30

Model studies on radical induced dna cleavage associated with the c-1' position

Cel



Currently, there has been considerable interest in the mechanisms associated with radical-induced DNA cleavage. Apart from the established intermediacy of radicals in the C4' and C-5' positions of the sugar backbone, there has been substantial experimental evidence that C-l' radicals are also intermediates in the reaction between neocarzinostatin and double stranded DNA containing dAGC sequences and also in the reaction of bleomycin with oligonucleotides modified with a single ribo or arabino nucleoside. The goals of the proposed research will be to prepare a number of modified nucleosides and oligonucleotides, with modifications that can generate specifically a single C-1' radical species and study its degradation under a variety of condiiions. Moreover, we plan to design a second set of modifications which can specifically trap an in vitro generated C-1' radical species, this being either a carbon centered or oxygen radical.

Dziedzina nauki (EuroSciVoc)

Program(-y)

Temat(-y)

Zaproszenie do składania wniosków

Brak dostępnych danych

System finansowania

RGI - Research grants (individual fellowships)

Koordynator

NATIONAL RESEARCH COUNCIL OF ITALY
Wkład UE
Brak danych
Adres
via Piero Gobetti 101
40129 BOLOGNA
Włochy
Koszt całkowity
Brak danych

Uczestnicy (1)