Objectif SUMMARY All mammalian mRNAs end with a 200 adenosine residues tail at their 3' ends. Recently several studies have highlighted the role of the poly(A) tail in translation and mRNA stability. A detailed biochemical characterization of the protein components involved in the nuclear polyadenylation have started during last years. Poly(A) polymerase (PAP), necessery for the adenosine addition reaction, has been molecularly cloned from bovine and human. The availability of purified polyadenylation factors and monoclonal antibodies (Mabs) directed against human PAP makes it possible to generate an epitope map of PAP describing the relative location in space of the different epitopes. The epitope map will be related to a linear map which will be obtained by deletion mutagenesis of recombinant PAP followed by Western blot analysis of the various modified PAPs. Comparison of the linear functional maps with the tentative folding map, will allow us to predict a detailed functional/ structural map of PAP. The identification of different epitopes of PAP and their function, will help us to understand the significance of multiple PAPs in mammalian cells. Champ scientifique sciences naturellessciences chimiqueschimie organiqueréaction organiquesciences naturellessciences biologiquesbiochimiebiomoléculeprotéines Programme(s) FP4-TMR - Specific research and technological development programme in the field of the training and mobility of researchers, 1994-1998 Thème(s) 0302 - Post-doctoral research training grants TL02 - Molecular Biology and Biochemistry Appel à propositions Data not available Régime de financement RGI - Research grants (individual fellowships) Coordinateur Uppsala University Adresse 3,husargatan 751 23 Uppsala Suède Voir sur la carte Contribution de l’UE Aucune donnée Participants (1) Trier par ordre alphabétique Trier par contribution de l’UE Tout développer Tout réduire Not available Grèce Contribution de l’UE € 0,00 Adresse Voir sur la carte Autres sources de financement Aucune donnée
