Objetivo The coenzyme pyridoxal phosphate mediates in the catalysis of metabolic reactions of D- and L- amino acids. Although much work has been done on the mechanism of action and stereospecificity of these processes, it has been difficult to examine the intermediate stages because the substrates are processed so effectively. We therefore propose to synthesise stereospecifically labelled inhibitors of these enzymes and use them to study the overall mechanism of action of the reactions. This will involve isolation of enzyme-inhibitor complexes and trapping of enamine intermediates with thiols. The stereospecificity of formation of the Snackertz intermediate will also be studied. The use of these inhibitors will allow intimate details of the mechanism to be studied. The involvement of D-amino acids in bacterial metabolic processes makes the enzyme inhibitors of potential value as anti-bacterial drugs. Ámbito científico natural scienceschemical sciencescatalysisnatural scienceschemical sciencesorganic chemistryaminesnatural sciencesbiological sciencesbiochemistrybiomoleculesproteinsenzymes Programa(s) FP4-TMR - Specific research and technological development programme in the field of the training and mobility of researchers, 1994-1998 Tema(s) 0302 - Post-doctoral research training grants TC03 - Biological, Medicinal and Pharmaceutical Chemistry Convocatoria de propuestas Data not available Régimen de financiación RGI - Research grants (individual fellowships) Coordinador University of Sussex Aportación de la UE Sin datos Dirección Falmer BN1 9QJ Brighton Reino Unido Ver en el mapa Coste total Sin datos Participantes (1) Ordenar alfabéticamente Ordenar por aportación de la UE Ampliar todo Contraer todo Not available Irlanda Aportación de la UE Sin datos Dirección Ver en el mapa Coste total Sin datos
