Objectif Research objectives and content Despite the importance of gliogenesis in nervous system morphogenesis, the molecular mechanisms of glial differentiation have been poorly investigated. glide/gcm is a D. melanogaster gene expressed and required in glial cells. The project is aimed to determine the role of glide/gcm in gliogenesis and the genes regulating glide/gcm activity. To this purpose the effect of ubiquitous and cell type specific ectopic expression of glide/gcm will be tested. The definition In vivo of the minimal sequences necessary for glide/gcm activity will be obtained by rescue of the mutant phenotype via germ-line transformation. Finally I will perform a mutagenesis screen aimed to identify genes interacting genetically with glide/gcm by scoring for mutations that enhance or suppress the glide/gcm phenotype. Training content (objective, benefit and expected impact) The research project I propose will enable us to determine how glide/gcm induces gliogenesis and how its activity is regulated. To better understand the molecular bases of glial cell differentiation. The proposed project implies the use of different approaches in molecular biology and genetics. The possibility of carrying on the project in an institute of outstanding quality such as the IGBMC constitutes a unique opportunity to broaden my knowledge and skills in developmental biology and neurobiology and I consider that such training is fundamental for my research career. Champ scientifique natural sciencesbiological sciencesneurobiologynatural sciencesbiological sciencesdevelopmental biologynatural sciencesbiological sciencesgeneticsmutationnatural sciencesbiological sciencesmolecular biology Programme(s) FP4-TMR - Specific research and technological development programme in the field of the training and mobility of researchers, 1994-1998 Thème(s) 0302 - Post-doctoral research training grants TL06 - Developmental Biology Appel à propositions Data not available Régime de financement RGI - Research grants (individual fellowships) Coordinateur CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE Contribution de l’UE Aucune donnée Adresse Rue Laurent Fries 1 67404 ILLKIRCH GRAFFENSTADEN France Voir sur la carte Coût total Aucune donnée Participants (1) Trier par ordre alphabétique Trier par contribution de l’UE Tout développer Tout réduire Not available Italie Contribution de l’UE Aucune donnée Adresse Voir sur la carte Coût total Aucune donnée