Objective
Research objectives and content
The interest of this research work is directed to study DNA polymers and oligomers containing A-T base pairs by X-ray diffraction. The two main objectives consist in determining the structure of A-T rich regions in DNA double helices and in understanding the interaction of these DNA sequences with basic proteins. This alternating sequence is also essential for DNA trascription, since it is the essential element of the TATA box. This project aims to understand further the versatility of (TA)n sequences, in particular when they interact with non-specific proteins such as protamines and histones.
The first part of the research work consists in searching the D form of the following sequences by single crystal X-ray diffraction; trying to find adequate crys-allization conditions, which should promote the D form. The second part consists in co-crystallizing these fragments with basic peptides. The peptide sequences are consensus sequences of histone Hl and some basic sperm proteins.
Besides this, the CGTATATACG refinament structure represents another important objective in a short time.
Training content (objective, benefit and expected impact)
Objective: To obtain the Ph.D degree; Benefit: To learn Macromolecular Crystallography; Expected Impact: Find new structures of DNA, improving knowledge about relation between topology and function.
Links with industry / industrial relevance (22)
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences genetics DNA
- natural sciences earth and related environmental sciences geology mineralogy crystallography
- natural sciences mathematics pure mathematics topology
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences chemical sciences polymer sciences
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
08028 BARCELONA
Spain
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.