Role of adam 12 in muscle regeneration

Informations projet

N° de convention de subvention: HPMF-CT-2002-01557

Projet clôturé

Date de début 1 Janvier 2002

Date de fin 30 Juin 2003

Financé au titre de

• Programme for research, technological development and demonstration on "Improving the human research potential and the socio-economic knowledge base" (1998-2002)

Coût total € 108 514,00

Contribution de l’UE € 108 514,00

Coordonné par

UNIVERSITY OF COPENHAGEN
Denmark

Objectif

We have previously found that ADAM 12 (A Disintegrin And Metalloprotease) can provoke myogenesis and is upregulated during muscle regeneration, but the mechanisms involved are unknown. We have generated transgenic mice over expressing ADAM 12 and bred them to mdx mice, which are the animal model for Duchenne muscular dystrophy. Interestingly, we found a significantly decreased degree of muscle cell necrosis and a decreased level of serum creatine kinase in these ADAM 12/mdx mice compared to mdx mice. Based on these striking results / we are interested in identifying molecular pathways by which ADAM 12 is implicated in myogenesis and muscle regeneration. For this purpose we will use the microarray technology to study the expression profile of 12000 genes in normal, ADAM 12 transgeneic, mdx, and mdx/ADAM 12 transgenic mice. Genes which expression is significantly modulated will be classified in functional clusters, which should give rise to different hypotheses concerning the molecular pathways of ADAM 12 action in muscle regeneration. We will then use different functional assays to test these hypotheses. Based on our preliminary results, we hypothesize that ADAM12 might contribute to muscle cell survival by playing an anti-apoptotic role. To test this hypothesis we will first focus on the analysis of calcium signaling which is known to be implicated in anti-apoptotic signals. As the analysis of the modulated genes goes on, other hypotheses might rise and will be tested.

Champ scientifique (EuroSciVoc)

CORDIS classe les projets avec EuroSciVoc, une taxonomie multilingue des domaines scientifiques, grâce à un processus semi-automatique basé sur des techniques TLN.

• sciences naturelles › sciences chimiques › chimie inorganique › métal alcalinoterreux
• sciences médicales et de la santé › médecine fondamentale › neurologie › dystrophie musculaire › myopathie de duchenne

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Programme(s)

• FP5-HUMAN POTENTIAL - Programme for research, technological development and demonstration on "Improving the human research potential and the socio-economic knowledge base" (1998-2002)

Thème(s)

Data not available

Appel à propositions

Data not available

Régime de financement

RGI - Research grants (individual fellowships)

Coordinateur

UNIVERSITY OF COPENHAGEN

• Contribution de l’UE: Aucune donnée
• Adresse: Frederik V's vej 11 ; 1017 KOEBENHAVN K/COPENHAEGEN ; Danemark
• Coût total: Aucune donnée