Role of adam 12 in muscle regeneration

Informazioni relative al progetto

ID dell’accordo di sovvenzione: HPMF-CT-2002-01557

Progetto chiuso

Data di avvio 1 Gennaio 2002

Data di completamento 30 Giugno 2003

Finanziato da

• Programme for research, technological development and demonstration on "Improving the human research potential and the socio-economic knowledge base" (1998-2002)

Costo totale € 108 514,00

Contributo UE € 108 514,00

Coordinato da

UNIVERSITY OF COPENHAGEN
Denmark

Obiettivo

We have previously found that ADAM 12 (A Disintegrin And Metalloprotease) can provoke myogenesis and is upregulated during muscle regeneration, but the mechanisms involved are unknown. We have generated transgenic mice over expressing ADAM 12 and bred them to mdx mice, which are the animal model for Duchenne muscular dystrophy. Interestingly, we found a significantly decreased degree of muscle cell necrosis and a decreased level of serum creatine kinase in these ADAM 12/mdx mice compared to mdx mice. Based on these striking results / we are interested in identifying molecular pathways by which ADAM 12 is implicated in myogenesis and muscle regeneration. For this purpose we will use the microarray technology to study the expression profile of 12000 genes in normal, ADAM 12 transgeneic, mdx, and mdx/ADAM 12 transgenic mice. Genes which expression is significantly modulated will be classified in functional clusters, which should give rise to different hypotheses concerning the molecular pathways of ADAM 12 action in muscle regeneration. We will then use different functional assays to test these hypotheses. Based on our preliminary results, we hypothesize that ADAM12 might contribute to muscle cell survival by playing an anti-apoptotic role. To test this hypothesis we will first focus on the analysis of calcium signaling which is known to be implicated in anti-apoptotic signals. As the analysis of the modulated genes goes on, other hypotheses might rise and will be tested.

Campo scientifico (EuroSciVoc)

CORDIS classifica i progetti con EuroSciVoc, una tassonomia multilingue dei campi scientifici, attraverso un processo semi-automatico basato su tecniche NLP. Cfr.: https://op.europa.eu/en/web/eu-vocabularies/euroscivoc.

• scienze naturali › scienze chimiche › chimica inorganica › metalli alcalino terrosi
• scienze mediche e della salute › medicina di base › neurologia › distrofie muscolari › distrofia muscolare di duchenne

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Programma(i)

• FP5-HUMAN POTENTIAL - Programme for research, technological development and demonstration on "Improving the human research potential and the socio-economic knowledge base" (1998-2002)

Argomento(i)

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Meccanismo di finanziamento

RGI - Research grants (individual fellowships)

Coordinatore

UNIVERSITY OF COPENHAGEN

• Contributo UE: Nessun dato
• Indirizzo: Frederik V's vej 11 ; 1017 KOEBENHAVN K/COPENHAEGEN ; Danimarca
• Costo totale: Nessun dato