Objectif Foreseen ResultsThe goals 1-3 will generate information that will be of general importance for the efficient expression of foreign proteins in transgenic plants. The goal 4 will generate virus expression vectors that will be generally useful in plant molecular plants. These vectors will allow high level expression of foreign proteins in plants without the need to resort to time consuming transformation technology. The sobemovirus vectors will also have practical importance that will be tested in this project by the expression of vaccine proteins in infected plants. This work reflects a novel approach to the production of oral vaccines for use ultimately in both animals and humans. The final goals 5 and 6 will lead to the production of virus resistant plants and are a potential solution to the disease problems posed by the two viruses used in this project. The recent development of transformation technology in monocotyledonous plants means that the transgenic resistance is now a feasible target of plant biotechnology. The analysis of natural resistance will have a longer term benefit in the production of plants resistant to sobemoviruses and other pathogens.The proposed work is a molecular analysis of cocksfoot mottle virus and rice yellow mottle virus. These are both members of the sobemovirus group and are pathogens of fodder crops and rice respectively. The programme has several related goals. These include:1. The characterization of structure and functions of various sobemovirus proteins;2. The understanding of translational strategies exploited by sobemoviruses;3. The identification of promoters for the subgenomic RNA synthesis and translational enhancers and the evaluation of their potential as tools for the construction of efficient plant expression vectors;4 Construction of transient plant expression vectors based on sobemoviral sequences and their use of the expression of foreign sequences, including vaccine antigens;5. Analysis of natural resistance against different sobemoviruses and the identification of corresponding resistance genes;6. Construction of transgenic plants expressing sobemovirus-derived sequences and the use of these plants for the studies on sobemovirus proteins functions and as the source for the genetically engineered resistance against sobemovirus infections. Champ scientifique natural sciencesbiological sciencesmicrobiologyvirologynatural sciencesbiological sciencesbiochemistrybiomoleculesproteinsmedical and health sciencesbasic medicinepharmacology and pharmacypharmaceutical drugsvaccinesagricultural sciencesagriculture, forestry, and fisheriesagricultureindustrial cropsfoddernatural sciencesbiological sciencesgeneticsRNA Programme(s) FP4-INCO - Specific research, technological development and demonstration programme in the field of cooperation with third countries and international organizations, 1994-1998 Thème(s) 01020902 - Plant molecular and cellular biology Appel à propositions Data not available Régime de financement CSC - Cost-sharing contracts Coordinateur THE SAINSBURY LABORATORY Contribution de l’UE Aucune donnée Adresse John Innes CentreNorwich Research Park, Colney NR4 7UH NORWICH Royaume-Uni Voir sur la carte Coût total Aucune donnée Participants (4) Trier par ordre alphabétique Trier par contribution de l’UE Tout développer Tout réduire Institute of Agricultural Biotechnology Russie Contribution de l’UE Aucune donnée Adresse 42,Timiryazevskaya Street 127550 Moscow Voir sur la carte Coût total Aucune donnée Latvijas Universitate Lettonie Contribution de l’UE Aucune donnée Adresse 1,Ratsupites Street 1067 Riga Voir sur la carte Coût total Aucune donnée NATIONAL INSTITUTE OF CHEMICAL PHYSICS AND BIOPHYSICS Estonie Contribution de l’UE Aucune donnée Adresse 23,Akademia Tee 23 12618 TALLINN Voir sur la carte Coût total Aucune donnée UNIVERSITY OF HELSINKI Finlande Contribution de l’UE Aucune donnée Adresse 9,Viikinkaari 9 Biocentre 1A 00014 Helsinki Voir sur la carte Coût total Aucune donnée