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Contenido archivado el 2024-06-11

Identification and characterisation of Malaria vectors in Southeast Asia: a prerequisite for appropriate vector-control

Objetivo

General objectives

* To develop in Southeast Asia appropriate vector control measures based on the correct identification of the vector species involved in malaria transmission and on their behaviour in order to reduce malaria incidence in populations at high risk and to prevent malaria epidemics by early detection and control.

Measurable specific objectives

* To elucidate the epidemiological relevance of heterogeneities in vector populations and the sibling species of malaria vectors in different physico-geographical regions of Southeast Asia.
* To develop improved methods for vector species identification and to obtain clear criteria that can be used to define malaria risk areas; this will help in the choice and implementation of preventive measures.
* To study the practical implications of the genetically defined sibling species in relation to vector behaviour, vectorial capacity.
* Capacity building by exchange of technologies and collaborative training in research methodology.
Expected Outcome

The project will assess the species status of the three major malaria vectors in Southeast Asia and will evaluate the role of the individual species in malaria transmission. This will lead to considerable improvement of the vector control activities by focusing the control measures on the efficient vectors with the appropriate tools and in relation to their behaviour. Moreover, a better evaluation of the intervention, using the new identification methods, is an additional benefit. It would also be possible to estimate the risk of environmental changes on vector distribution.//REF PJ-11684
The study will be performed in three physico-geographical regions of Vietnam (northern, central and southern), in Laos and in Cambodia.
Baseline ecological and malaria epidemiological data will be collected for each study site.
Mosquitoes (adults and larvae) will be collected in each study site applying multiple collection methods during the first year of the project.
Different experimental procedures to analyse the field samples will be applied: morphology, isozyme electrophoresis, RAPD-PCR, PCR-RFLP and ELISA.

Isozyme electrophoresis will be applied to morphologically identified forms (morphotypes) in order to confirm species identification, and to discriminate individual members of species complexes. This technique will provide information on the extent of genetic variation between and within different populations. This will allow us to detect intrinsic barriers to gene flow by the demonstration of a lack of heterozygotes. The enzymatic markers will be tested for their ability to distinguish potential cryptic species and subsequently used to investigate species relationships relevant to vector biology and transmission.

Molecular techniques will be applied for the development of simple and reliable tools for species identification; e.g. screening of RAPD-PCR primers; amplification of variable rDNA regions and RFLP analysis. The molecular markers will be tested for their ability to distinguish cryptic species identified by isozyme electrophoresis.

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Coordinador

Instituut voor Tropische Geneeskunde Prins Leopold
Aportación de la UE
Sin datos
Dirección
155,Nationalestraat
2000 Antwerpen
Bélgica

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