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Virological and molecular characteristics of HIV strains in Africa: the impact of genetic subtypes on the pathogenesis and prognosis of HIVinfection

Objective

General objective

To analyse the impact of specific genetic subtypes on the natural history of HIV-1 infection.

Specific objective

* To identify the genetic subtype circulating in three existing cohorts of HIV infected patients in Senegal, Cameroon and Guinea-Bissau; to study virological and antigenic characteristics related to the genotype.
* To identify risk factors associated to specific genotypes and to assess clinical, immunological and virological parameters as well as survival over 3 years period in HIV-1 infected patients according to genotypes.
Expected Outcome

The potential benefits of the proposed projects are multiple. A better clinical care of HIV infected patients is offered through a standardized approach and with the possibility to treat infections due to the immuno-depression.
This project consists on two parts:

First, a transversal study among ongoing cohorts of HIV infected patients in Senegal, Cameroon and Guinea-Bissau will allow to determine the prevalence of the different genotypes circulating in this populations and to characterize their virological properties. Both heteroduplex mobility assay (HMA) and V3 peptide serology will be used to diagnose the different genetic groups and subtypes of HIV-1. An algorithm will be developed to simplify the genetic subtyping of HIV-1. The biological phenotype will be analyzed and correlated with the genetic sequence of the V3 loop. Risk factors associated to genotypes will be analyzed.

Secondly, the possibility to perform a follow-up of HIV-infected patients in Senegal, Guinea-Bissau and Cameroon allows us to initiate a prospective study in order to assess clinical, immunological and virological parameters as well as survivial over a three year period. The biological phenotype will be determined every 6 months and correlated with CD4 counts and clinical parameters. Several clinical, serological, virological and immunological parameters will be studied in order to see if certain genotypes have a different pathogenesis and prognosis for HIV disease development.

Funding Scheme

CSC - Cost-sharing contracts

Coordinator

Institut de Recherche pour le Développement
Address
Avenue Agropolis 911
34032 Montpellier
France

Participants (6)

Centre Hospitalier et Universitaire
Cameroon
Address

10 Yaounde
Innogenetics NV
Belgium
Address
7,Industriepark Zwijnaarde
9052 Zwijnaarde
Institut de Médecine et d'Epidémiologie Africaines et Tropicales
France
Address
16,Rue Henri Huchard
75018 Paris
STATENS SERUM INSTITUT
Denmark
Address
5,Artillerivej 5
2300 Koepenhagen
UNIVERSITE CHEIKH ANTA DIOP DE DAKAR
Senegal
Address
Avenue Pasteur
30 Dakar
Université François Rabelais de Tours
France
Address
2,Boulevard De La Tonelle
37044 Tours