Objective
* The development of rDNA-based molecular markers for each of the taxonomic units shown within An. gambiae s.s. and An. funestus s.s.
* The evaluation of the new diagnostic tools on field-collected material typed by polytene chromosome analysis.
* The physical isolation and cloning of the DNA surrounding the breakpoints of inversion 2La.
* The sequence determination of specific DNA segments linked to 2La and their use in the karyotype identification of field-collected malaria vectors and in phylogenetic studies.
Expected Outcome
* The development of rDNA-based molecular markers for each of the taxonomic units shown within An. gambiae s.s. and An. funestus s.s.
* The evaluation of the new diagnostic tools on field-collected material typed by polytene chromosome analysis.
* The physical isolation and cloning of the DNA surrounding the breakpoints of inversion 2La.
* The sequence determination of specific DNA segments linked to 2La and their use in the karyotype identification of field-collected malaria vectors and in phylogenetic studies.
This proposal focuses on the molecular analysis of the Anopheles gambiae and the An. funestus complexes, the malaria vector system in Sub-Saharan Africa. The sibling species in the complexes are defined by the presence or absence of specific inversions detected on polytene chromosomes. Furthermore, there are indications that additional taxa carrying typical sets of polymorphic chromosomal inversions and characterized by variable degrees of intergradation, are members of these complexes. The long term goal is to undertake an in depth study of the genetic structure and dynamics of field populations of An. gambiae and An. funestus and to attempt to correlate specific recognizable genetic polymorphisms with vector biology, in order to provide a better description of this powerful vector system and to obtain reliable data towards its monitoring and control.
We propose to undertake a molecular genetic analysis of the inversion polymorphisms using molecular probes. The primary objective of the present study is the development of specific PCR-based diagnostics based on both rDNA (for both An. gambiae and An. funestus) and cloned inversion breakpoints for the 2La inversion of An. gambiae. As a parallel objective, we wish to analyze the phylogenetic relationship of chromosomal forms of An. gambiae and to obtain information on the monophyletic origin of the inversions and on introgression and gene flow among different inversions.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- medical and health sciences health sciences infectious diseases malaria
- natural sciences biological sciences genetics DNA
- natural sciences biological sciences genetics chromosomes
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Coordinator
71110 IRAKLION
Greece
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