New design strategies for interactive antisense oligonucleotides for potential therapeutic application under physiological conditions

Projektinformationen

ID Finanzhilfevereinbarung: INTAS-93-0828

Startdatum 1 November 1994

Enddatum 31 Oktober 1995

Finanziert unter

• International Association for the promotion of cooperation with scientists from the independent states of the former Soviet Union (INTAS), 1993-

Gesamtkosten € 0,00

EU-Beitrag € 18 000,00

Koordiniert durch

Universität Ulm
Germany

Ziel

The aim of this project is the design of interactive antisense oligonucleotides for therapeutic use at physiological temperatures. This requires that the specificity of hybridisation be maintained and the thermal behaviour, i.e. the discriminatory temperature range near melting, adapted to physiological conditions.

As a route to achieving this goal, chimeric oligonucleotide-oligoethylene glycol oligoether-phosphates are prepared. In these chimeric compounds, the oligonucleotide segments will be short enough to ensure melting temperatures near physiological, whereas the total length of base pairing will be maintained around 17-20 bases to ensure maximum specificity. The oligoethylene glycol moieties will not only serve as spacers between the oligonucleotide segments, but may also enhance the cellular uptake of these macromolecules.

The conjugation to organic substituents will be studied as follows: modulate oligonucleotide hybridisation (e.g. phenazinium residues); account for improved uptake, and produce cytotoxic effects in target cells by, non-triggered interaction with nucleic acids through alkylating residues or by triggered cleavage of nucleic acids by hydrophilic porphine moieties.

Programm/Programme

• IC-INTAS - International Association for the promotion of cooperation with scientists from the independent states of the former Soviet Union (INTAS), 1993-

Thema/Themen

• 36 - Biochemistry and New Pharmaceutical Compounds

Aufforderung zur Vorschlagseinreichung

Data not available

Finanzierungsplan

Data not available

Koordinator

Universität Ulm

• EU-Beitrag: Keine Daten
• Adresse: Albert-Einstein-Allee 11 ; 89069 Ulm ; Deutschland
• Gesamtkosten: Keine Daten

Beteiligte (2)

Novosibirsk Institute of Bioorganic Chemistry

Russland

• EU-Beitrag: Keine Daten
• Adresse: 630090 Novosibirsk
• Gesamtkosten: Keine Daten

UNIVERSITA DEGLI STUDI DI PADOVA

Italien

• EU-Beitrag: Keine Daten
• Adresse: Via F. Marzolo 1 ; 35131 PADOVA
• Gesamtkosten: Keine Daten