Objective Lymphokine-activated killer (LAK) cells have high anti-tumour activity against autologous and allogenic tumour cells. This cell mediated cytotoxicity is due both to secreted and to membrane-associated cytotoxic proteins. The tumour necrosis factor, lymphotoxin and gamma-interferon, contribute to the cytotoxicity of LAK cells but other cytotoxic factors from LAK cells have not been characterised. The cytotoxic proteins from LAK cells will be purified in order to determine their partial amino acid sequence and then to clone their genes. Primary structure, immunoreactivity and biological activity of the purified proteins (induction of necrotic or apoptotic pathways of target cells death, specificity to different target cells, etc.) will be compared with the tumour necrosis factor. The results obtained will be valuable for use in continuing research to elucidate the molecular mechanism of anti-tumour activity of cytotoxic lymphocytes and may find applicability in the development of treatment of tumour diseases. Programme(s) IC-INTAS - International Association for the promotion of cooperation with scientists from the independent states of the former Soviet Union (INTAS), 1993- Topic(s) 44 - Biotechnology Call for proposal Data not available Funding Scheme Data not available Coordinator International Centre for Genetic Engineering and Biotechnology EU contribution No data Address Padriciano 99 34012 Trieste Italy See on map Total cost No data Participants (2) Sort alphabetically Sort by EU Contribution Expand all Collapse all Russian Academy of Sciences Russia EU contribution No data Address 117334 Moscow See on map Total cost No data Universiteit Gent,Vakgroep Biochemie Belgium EU contribution No data Address 9000 Gent See on map Total cost No data
