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Content archived on 2022-12-23

Biochemical and genetic characterisation of Chlamydomonas reinhardii mutants

Objective



Biochemical characterisation of mutant strains is a prerequisite to successful studies in the field of molecular genetics. Chlamydomonas reinhardii, a green alga, is a model object for modern cell and molecular biology, especially in the field of photosynthesis.

In this project, carotenoid-deficient, chlorophyll-deficient, and norflurazon-resistant mutants will be studied to learn more about biosynthesis of photosynthetic structures and photoprotection of the photosynthetic apparatus. Successful search for carotenoid precursors has been carried out in Bern in several Chlamydomonas reinhardii mutants. Further studies of the mutants should proceed towards biochemical identification of biosynthetic precursors, which accumulate in the mutants, and towards genetic analysis of the mutants.

This goal will be best achieved by co-operation of different laboratories. The St. Petersbyrg group willprovide the mutants.

In St. Petersburg the genetic analysis, and in Bern some biochemical and photoprotective studies will be performed. In Freiburg the effect of light on hsp70 induction, and in Munich the synthesis of chlorophyll precursors in mutants will be analysed in order to find out how gene activity regulates chlorophyll biosynthesis and chloroplast biogenesis, and whether chlorophyll precursors are involved in the induction of hsp70 synthesis by light.

Programme(s)

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Topic(s)

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Call for proposal

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Funding Scheme

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Coordinator

Universität Bern
EU contribution
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Address
Freiestraße 3
3012 Bern
Switzerland

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Total cost

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Participants (3)

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