CORDIS provides links to public deliverables and publications of HORIZON projects.
Links to deliverables and publications from FP7 projects, as well as links to some specific result types such as dataset and software, are dynamically retrieved from OpenAIRE .
Exploitable results
An essential step in the biosynthesis of coloured carotenoids consists in the introduction of the conjugated double bond system. The first enzyme responsible is phytoene desaturase which can be inhibited by norflurazone. By studying a number of norflurazone resistant mutants (nfr) it could be shown that at least 3 nuclear genes are involved in phytoene dehydrogenation affecting phytoene accumulation and resistance level. The next enzyme in carotenoid biosynthesis in green plants and algae is a zeta-carotene dehydrogenase. Based on previous experiments of A.S.Chunayev, different strains accumulating zeta-carotene in the dark could be ascribed to mutations of the ac5-gene.
The role of the different stereoisomeres of zeta-carotene in these mutants is not yet known. Furthermore, mutants with altered chlorophyll biosynthesis have also been studied. From the well characterised chlorophyll b deficient mutants (cbn) revertants have been induced. By genetic crossings and pigment analysis of segregants the existence of several independent suppresser genes (sub) was demonstrated. Also mutants with defects early in the chlorophyll biosynthetic pathway have been characterised genetically and by measuring the accumulation of pigment precursors. In this context, an interesting new phenotype related to a uniparentally, eventually chloroplastidially inherited gene was described (mod-u-25). Mutation of this gene resulted in cells with different colours, ALA synthesising activity and pigment composition. Based on these characterisations of pigment mutants, molecular genetic experiments should now be designed for future research on the enzymes involved in pigment biosynthesis in Chlamydomonas.
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