Biochemical and genetic characterisation of Chlamydomonas reinhardii mutants

Informacje na temat projektu

Identyfikator umowy o grant: INTAS-94-2594

Projekt został zamknięty

Data rozpoczęcia 1 Kwietnia 1995

Data zakończenia 31 Marca 1997

Finansowanie w ramach

• International Association for the promotion of cooperation with scientists from the independent states of the former Soviet Union (INTAS), 1993-

Koszt całkowity

Brak danych

Wkład UE

€ 26 000,00

Koordynowany przez

Universität Bern
Switzerland

CORDIS oferuje możliwość skorzystania z odnośników do publicznie dostępnych publikacji i rezultatów projektów realizowanych w ramach programów ramowych HORYZONT.

Odnośniki do rezultatów i publikacji związanych z poszczególnymi projektami 7PR, a także odnośniki do niektórych konkretnych kategorii wyników, takich jak zbiory danych i oprogramowanie, są dynamicznie pobierane z systemu OpenAIRE .

Wyniki nadające się do wykorzystania

Biochemical and genetic characterisation of Chlamydomonas reinhardii mutants

An essential step in the biosynthesis of coloured carotenoids consists in the introduction of the conjugated double bond system. The first enzyme responsible is phytoene desaturase which can be inhibited by norflurazone. By studying a number of norflurazone resistant mutants (nfr) it could be shown that at least 3 nuclear genes are involved in phytoene dehydrogenation affecting phytoene accumulation and resistance level. The next enzyme in carotenoid biosynthesis in green plants and algae is a zeta-carotene dehydrogenase. Based on previous experiments of A.S.Chunayev, different strains accumulating zeta-carotene in the dark could be ascribed to mutations of the ac5-gene. The role of the different stereoisomeres of zeta-carotene in these mutants is not yet known. Furthermore, mutants with altered chlorophyll biosynthesis have also been studied. From the well characterised chlorophyll b deficient mutants (cbn) revertants have been induced. By genetic crossings and pigment analysis of segregants the existence of several independent suppresser genes (sub) was demonstrated. Also mutants with defects early in the chlorophyll biosynthetic pathway have been characterised genetically and by measuring the accumulation of pigment precursors. In this context, an interesting new phenotype related to a uniparentally, eventually chloroplastidially inherited gene was described (mod-u-25). Mutation of this gene resulted in cells with different colours, ALA synthesising activity and pigment composition. Based on these characterisations of pigment mutants, molecular genetic experiments should now be designed for future research on the enzymes involved in pigment biosynthesis in Chlamydomonas.