The objective of this proposal is to characterise differentially displayed (dd) proteins and mRNAs whose expression patterns are specific to cystic fibrosis (CF) in order to get a better understanding of the mechanisms underlying this disease, and to improve diagnosis and/or therapy. CF is the most common lethal monogenic disease in Europe. It is recognised that CFTR, the key protein whose dysfunction causes CF, is at the crossroad of important pathways and plays major roles, most of them unknown, within a network of proteins central to a whole series of cellular functions. As a result, not only genetic predisposition but also many environmental and/or pharmacological factors can affect CF severity. So far, the reponses of CF to these various factors have been studied in a limited context such as, for instance, one gene or pathway at a time. Thus studies of proteomics of CF are needed. We will use high performance techniques to analyse the protein patterns from cells of CF patients vs. non-affected individuals and will establish a database of dd-proteins and mRNAs. Our new techniques for proteome analysis will be useful for other applications.
Funding SchemeCSC - Cost-sharing contracts
WC1E 6JJ London