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Development of ultrasensitive methods for proteome: application to cystic fibrosis (EUROPROCF)

Objective

The objective of this proposal is to characterise differentially displayed (dd) proteins and mRNAs whose expression patterns are specific to cystic fibrosis (CF) in order to get a better understanding of the mechanisms underlying this disease, and to improve diagnosis and/or therapy. CF is the most common lethal monogenic disease in Europe. It is recognised that CFTR, the key protein whose dysfunction causes CF, is at the crossroad of important pathways and plays major roles, most of them unknown, within a network of proteins central to a whole series of cellular functions. As a result, not only genetic predisposition but also many environmental and/or pharmacological factors can affect CF severity. So far, the reponses of CF to these various factors have been studied in a limited context such as, for instance, one gene or pathway at a time. Thus studies of proteomics of CF are needed. We will use high performance techniques to analyse the protein patterns from cells of CF patients vs. non-affected individuals and will establish a database of dd-proteins and mRNAs. Our new techniques for proteome analysis will be useful for other applications.

Funding Scheme

CSC - Cost-sharing contracts

Coordinator

INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE
Address
Faculté De Médecine Necker - Enfants Malades, Rue
75730 Paris
France

Participants (7)

INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE
France
Address
Hôpital Henri Mondor, Avenue Du Maréchal De Lattre
94010 Creteil
INSTITUTE OF BIOCHEMISTRY AND BIOPHYSICS - POLISH ACADEMY OF SCIENCES
Poland
Address
36,Pawinskiego 5A
02-106 Warszawa
INSTITUTE OF BIOCHEMISTRY AND BIOPHYSICS - POLISH ACADEMY OF SCIENCES
Poland
Address
Pawinskiego 5A
02-106 Warszawa
NATIONAL RESEARCH INSTITUTE FOR MOTHER AND CHILD
Poland
Address
Kasprazaka, 17 A
01-211 Warsaw
PROTEOSYS AG
Germany
Address
Kupferberterrasse 17-19
55116 Mainz
UNIVERSITY COLLEGE LONDON
United Kingdom
Address
University Street 5
WC1E 6JJ London
UNIVERSITY OF COPENHAGEN
Denmark
Address
Blegdamsvej 3C, Bld.6.2.
2200 Koepenhagen