Characterization and utilization of two new filarial models for investigation of immunity, chemotherapy and pathology

Objective

CHARACTERIZIATION AND UTILISATION OF TWO NEW FILARIAL MODELS FOR INVESTIGATIONS OF IMMUNITY CHEMOTHERAPY AND PATHOLGY. THIS WORK IS CONCERNED WITH PREVENTION AND TREATMENT OF FILARIAL INFECTIONS AND IN PARTICULAR ANCHOCERIAS. ATTENTION WILL FOCUS ON THE INTERPLAY BETWEEN IMMUNITY, PATHOLOGY AND DRUG TREATMENT AND N PARTICULAR IVERMECETIN. KNOWLEDGE IN THIS AREA WILL BE IMPORTANT FOR THE SUCCESSFUL DEVELOPMENT AND USE OF A VACCINE. FOR THIS WORK TWO MURID MODELS WILL BE USED. FIRST LITOMOSOIDEDS SIGMODONTIS (STRAN PARIS) WHCIH CAN UDERGO COMPLETE CYCLICAL DEVELOPMENT IN IMMUUNICOMPENT MICE (MUSMUSCULUS) AND PROVIDES AN EXCELLENT OPPORTUNITY TO INVESTIGATE CELLULAR (AND HUMORAL) RESPONSES EVOKED BY A FILARIAL PARASITE IN THE BEST IMMUNOLOGICALLY CHARACTERIZED OF MAMMALIAN HOSTS. SECOND MONANEMA MARTINI WHICH PRESENTS WITH DERMAL MICROFILARIAE AND INDUCES OCULAR AND SKIN PATHOLOGY SIMILAR TO THAT SEEN IN HUMAN ONCHOCERCIASIS. THE OBJECTIVES ARE FURTHER PARASITOLIGAL CHARACTERIZATION OF THE FOLLOWING FILARIAL MODELS: L SINGMODONTIS IN MICE AND MERIONES AND M MARTINI IN LEMNISCOMY STRIATUS AND MERIONES. ASSESSMENT OF CELLULAR RESPONSES EVOKED BY L SIGMODONTIS IMMUNCOMPENTET MICE MUSMUSCULUS AND FOLLOWING VACCINATION WITH IRRADIATED IARVAE AND RECOMBINANT ANTIGENS. THIS INCLUDES AND INVESTIGATION OF THE ROLE OF THI AND TH2 T-HELPER CELL SUBSETS AND SELECTED CYCTOKINES. ASSESMENT OF THE EFFECT OF IVERMECTIN TREATMENT ON IMMUNE RESPONSE EVOKED BY L SIGMODONTIS AND M MARTINI. INCLUDING AN INVESTIGATION OF DRUG INDUCED IMMUNITY WHICH HAD BEEN OBSERVED TO FOLLOW IVERMECTIN TREATMENT IN MAN AND RODENT MODELS. THE PHARMACOKINETICS OF IVERMECITIN IN DIFFERENT RODENT MODELS WILL BE EXAMINED BEFORE AND AFTER INFECTION AND IMMUNIZATION. IDENTIFICATION OF POSSIBLE CORRELATIONS BETWEEN PARASITOLIGICAL FEATURES, DRUGS, IMMUNITY AND PATHOLOGY. THE TECHNIQUES PROCEDURES AND PERSONAL EXPERTISE REQUIRED EXIST IN THE LABOROTORIES OF THE FOUR PARTNERS. THIS PROGRAMME COMPLEMENTS OTHER COLLABORATIONS INCLUDING HUMAN FIELD STUDIES.

Programme(s)

• FP3-STD 3 - Specific research and technological development programme (EEC) in the field of the life sciences and technologies for developing countries, 1990-1994

Funding Scheme

CSC - Cost-sharing contracts

Coordinator

Naturalia et Biologia

Address

11 Place Mercelin Berthelot
75231 Paris
France

Participants (3)

THE CHANCELLOR, MASTERS AND SCHOLARS OF THE UNIVERSITY OF CAMBRIDGE

United Kingdom

Address

Trinity Lane, The Old Schools,
CB2 1TN Cambridge

THE UNIVERSITY OF TUEBINGEN

Germany

Address

27,Wilhelmstrasse 27
72074 Tuebingen

University of Nicaragua

Nicaragua

Address

Enugu