Objective
The aim of the research is the standardization of the analytical methodology of biogenic amines in fishery products.
Research was carried out in order to standardize the analytical methodology of biogenic amines in fishery products. This involved the comparison and standardization of analytical methods between reference laboratories and intercomparison exercises within the Community for the determination of biogenic amines in fish products and a description of standard operating procedures.
For histamine, the most important biogenic amine, putrescine, cadaverine and tyramine comparable results were obtained by the different methods used by the reference laboratories. About 90% of the biogenic amines were recovered from tuna and mackerel after sterilization.
The biogenic amines are stable in the tricarboxylic acid (TCA) extracts of fish during storage at a temperature range -20 C +20 C.
About 30% of the 35 European laboratories were able to determine histamine in fishery products (herring and tuna) at a (spiked) level of 25 to 110 mg/kg with an acceptable accuracy, repeatability and reproducibility using different analytical methods.
The accuracy and reproducibility of the methods, almost exclusively high performance liquid chromatography (HPLC), among the laboratories used for the other biogenic amines like putrescine, cadaverine and spermidine in fishery products are in general less satisfactory than those for histamine. The number of laboratories with an acceptable accuracy and an acceptable repeatability within the laboratory was rather small.
Standard operating procedures for the determination of biogenic amines in fishery products by CFA, HPLC (isocratic, gradient, precolumn and postcolumn derivatization) and IEC of the 4 reference laboratories were used.
The research project can be divided into 2 phases:
comparison and standardizing analytical methods between reference laboratories;
and intercomparison exercises within the Community for the determination of biogenic amines in fish products and description of standard operating procedures.
In the first phase, the 3 reference laboratories will compare and standardize their analytical methods for the determination of biogenic amines. Different high performance liquid chromatography (HPLC) methods and continuous flow methods for the determination of histamine and the other biogenic amines will be tested on standard solutions, appropriate extracts of different fish samples and on different fish samples with intrinsic and extrinsic labelled biogenic amines. At the same time, an inquiry will be organized into the methods for the determination of biogenic amines used in the fish processing industry, the fish and food inspection services, and the fish and food industry in order to evaluate the specifications needed for the standardized methods like accuracy, reproducibility, detection limit, rate of analysis etc.
The second phase will involve intercomparison exercises within the Community for the determination of biogenic amines in fish products and description of standard operating procedures. In several intercomparison exercises, samples from different fish species with different levels of biogenic amines will be analysed by the various laboratories of the fish processing industry, the fish and food inspection services and the fish and food research institutes within the countries in the Community as well as by the reference laboratories.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- agricultural sciences agriculture, forestry, and fisheries fisheries
- natural sciences biological sciences zoology ichthyology
- natural sciences chemical sciences organic chemistry amines
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Coordinator
1970 AD Ijmuiden
Netherlands
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