Objective Studies on long intergenic non-coding RNAs (lincRNAs) suggest an important role in development and cancer, however, functional lincRNAs in adult stem cells have been rarely defined. This project will study the role of lincRNAs in development of intestinal stem cell (ISC) one of the well identified adult stem cells. For this purpose, I will identify lincRNAs that are specifically expressed in ISCs by analyzing transcriptome of sorted ISCs. LincRNAs expected to regulate ISC development will be selected. I will investigate the mechanisms of action of the lincRNAs using in vitro cultured mini-gut. Lastly, I will elucidate the physiological function of the lincRNAs by generating knock-out mouse models.It has become evident that lincRNAs regulate multiple cellular functions including pluripotency, differentiation, and cell cycle. This project will, therefore, broaden our knowledge on adult stem cell biology revealing how lincRNAs play a role in self-renewal and differentiation of ISCs. At the same time, this project will extend our understanding of lincRNA biology by providing a comprehensive functional study in a physiologically relevant system. Furthermore, given that ISCs are highly linked with cancer stem cells, the defined lincRNAs might be used as diagnostic markers and therapeutic targets in cancer. Fields of science natural sciencesbiological sciencescell biologymedical and health sciencesmedical biotechnologycells technologiesstem cellsmedical and health sciencesclinical medicineoncologynatural sciencesbiological sciencesgeneticsRNA Programme(s) FP7-PEOPLE - Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) Topic(s) FP7-PEOPLE-2012-IIF - Marie Curie Action: "International Incoming Fellowships" Call for proposal FP7-PEOPLE-2012-IIF See other projects for this call Funding Scheme MC-IIF - International Incoming Fellowships (IIF) Coordinator KONINKLIJKE NEDERLANDSE AKADEMIE VAN WETENSCHAPPEN - KNAW EU contribution € 183 469,80 Address KLOVENIERSBURGWAL 29 HET TRIPPENHUIS 1011 JV AMSTERDAM Netherlands See on map Region West-Nederland Noord-Holland Groot-Amsterdam Activity type Research Organisations Administrative Contact Don Van Velzen (Mr.) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data
