Objective
Compaction is the earliest morphogenetic event in mouse embryonic development during which blastomeres increase the size of their cell-cell interfaces. This permits the establishment of apico-basal polarity and thereby the 1st fate specification of the blastomeres: trophectoderm derives from blastomeres with a large apical membrane at the cell-medium interface; whereas blastomeres composing the inner cell mass descend from cells with comparably larger basal cell-cell interfaces. Essential molecules regulating compaction were identified; among them are cell-cell adhesion and cytoskeletal components. However, the precise mechanism by which these molecules drive compaction is not yet understood. In particular, the forces and mechanical properties associated with adhesive and cytoskeletal components that are responsible for the embryo shape change remain uncharacterized. Combining biophysical methods and high-resolution microscopy, we aim to characterize cells mechanical properties and underlying molecules that control compaction. We will monitor, using micropipette aspiration techniques, the contractile and adhesive properties of mouse blastomeres during compaction. In addition, we will quantitatively track changes in cell shape and reorganization of key molecules. With this, we will build a spatio-temporal map of the mechanical properties during compaction. This interdisciplinary and quantitative approach will be key to build a comprehensive model of compaction in early mammalian development.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences developmental biology
- natural sciences physical sciences optics microscopy
- medical and health sciences clinical medicine embryology
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP7-PEOPLE-2012-IEF
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Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
69117 Heidelberg
Germany
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.