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Novel Cell Migration Assay Based on Microtissue Technology and Tissue-Specific Matrices

Objective

Cell migration assays are commonly used to study wound healing, cancer cell invasion, and tissue development. Problems associated with the gap closure assays typically employed are that:
(i) the stopper or scratch used to make the migration zone damages the extracellular matrix (ECM),
(ii) the migration zone size is limited by the size of the stopper, and
(iii) the scratched migration zone shapes and sizes are irreproducible. Cell migration is strongly coupled with the structure and mechanical properties of the ECM, and damage to the ECM alters the cell migration path.

The main objective of this project is to develop a prototype novel cell migration assay, which will significantly improve the predictive power of cell-based assays while avoiding problems associated with existing assays, based on seeding cells precisely on pristine extracellular matrix tissue mimics with native-like cell-functionality and reproducible migration zones.

In accomplishing this, we will also address the following questions:
• What are the structure-property relationships between collagen I matrices with controlled thicknesses and fibril diameter and alignment, and their mechanical and electromechanical properties?
• What are the critical parameters for achieving functional bonding between the substrate and the highly anisotropic viscoelastic collagen I matrices and controlling the overall mechanical properties?
• Does the distribution of collagen fibril polar ordering, i.e. piezoelectric domains, influence cell migration?
• What parameters control crimp formation in tendon-like collagen I matrices?
• What parameters control and explain the unusual viscoelastic properties (e.g. they not depend on the speed of deformation, at least within the interval 0.01 - 1 mm/sec) of tendon-like collagen matrices?
• Which cell types, including cancer cells, co-align with collagen fibril alignment or crimp direction?

Call for proposal

H2020-MSCA-RISE-2014
See other projects for this call

Coordinator

UNIVERSITY COLLEGE DUBLIN, NATIONAL UNIVERSITY OF IRELAND, DUBLIN
Address
Belfield
4 Dublin
Ireland
Activity type
Higher or Secondary Education Establishments
EU contribution
€ 216 000

Participants (5)

ABERYSTWYTH UNIVERSITY
United Kingdom
EU contribution
€ 310 500
Address
Visualisation Centre Penglais
SY23 3BF Aberystwyth
Activity type
Higher or Secondary Education Establishments
LOUGHBOROUGH UNIVERSITY
United Kingdom
EU contribution
€ 324 000
Address
Ashby Road
LE11 3TU Loughborough
Activity type
Higher or Secondary Education Establishments
ASHLAND SPECIALTIES IRELAND LIMITED

Participation ended

Ireland
EU contribution
€ 13 500
Address
National Science Park, Building V, Dublin Road, Petitswood
N91 F6PD Mullingar, Westmeath
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
MSC.SOFTWARE LTD.
United Kingdom
EU contribution
€ 27 000
Address
Msc House/lyon Way
GU16 7ER Frimley, Camberley Surrey
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
BLAFAR LIMITED
Ireland
EU contribution
€ 40 500
Address
1 Clonfadda Wood Mount Merriion Avenue
A94W1K6 Blackrock Dublin
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)

Partners (4)

FIBRALIGN CORPORATION
United States
Address
32930 Alvarado Niles Road Suite 350
94587 Union City Ca
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
INSPHERO AG
Switzerland
Address
Wagistrasse 27
8952 Schlieren
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
THE UNIVERSITY OF TEXAS SYSTEM
United States
Address
Colorado Street 601
78701 2982 Austin
Activity type
Higher or Secondary Education Establishments
GRIFFITH UNIVERSITY
Australia
Address
Kessels Road Nathan
4111 Brisbane
Activity type
Higher or Secondary Education Establishments