Objective Self-renew and multilineage potential characterize stem cells. We have recently described that pancreas progenitor cells extracted from adult donors can be expanded long-term in vitro into 3D structures, which we have termed organoids. Pancreas organoids reproduce in vitro all the features of pancreas ductal epithelia, and have a limitless expansion potential. Thus, pancreas organoids promise to boost cell therapy of type 1 diabetes. We have recently observed that progenitor cells organoids preserve their genetic stability over a long time in culture. That represents an advantage, when compared to iPS or hES derived approaches, where genetic instability raises concerns for their future therapeutic applications. While progenitor organoids are promising for the future of cell therapy, bringing stem cell-based therapies to patients requires a reliable characterization (“knowing what the cells do and how they do it”, i.e. a phenotypic and molecular biology characterization), chemically well-defined culture media, and the capacity of mass-production under GLP/GMP conditions.The LSFM4LIFE consortium aims to the mass production of pancreas organoids for the cellular therapy of type 1 diabetes. The goals of the project are: (1) optimize growth and differentiation of human pancreas stem-cell organoids by employing phenotypic and molecular high-throughput screening (2) standardize the growth and differentiation of the organoids under well-defined biochemical conditions, and (3) achieve GLP/GMP-production of the human organoids for preclinical studies and phase I clinical studies. The close collaboration in the consortium between academic researchers and industry, as well as its cross-disciplinary composition, are essential to realize the goals of the project. The work packages of the project will have a technological impact in the form of patents and first market replication. Fields of science medical and health sciencesbasic medicinepharmacology and pharmacypharmaceutical drugsmedical and health sciencesclinical medicineendocrinologydiabetesmedical and health sciencesmedical biotechnologycells technologiesstem cellsnatural sciencesphysical sciencesopticsmicroscopyconfocal microscopymedical and health sciencesclinical medicinetransplantation Keywords progenitor stem cells stem cell organoids type 1 diabetes cell therapy regenerative medicine Light Sheet-based Fluorescence Microscope LSFM high throughput screening HT-LSFM Programme(s) H2020-EU.3.1. - SOCIETAL CHALLENGES - Health, demographic change and well-being Main Programme H2020-EU.3.1.3. - Treating and managing disease Topic(s) PHC-16-2015 - Tools and technologies for advanced therapies Call for proposal H2020-PHC-2014-2015 See other projects for this call Sub call H2020-PHC-2015-two-stage Funding Scheme RIA - Research and Innovation action Coordinator JOHANN WOLFGANG GOETHE-UNIVERSITAET FRANKFURT AM MAIN Net EU contribution € 1 068 886,25 Address THEODOR W ADORNO PLATZ 1 60323 Frankfurt Am Main Germany See on map Region Hessen Darmstadt Frankfurt am Main, Kreisfreie Stadt Activity type Higher or Secondary Education Establishments Links Contact the organisation Opens in new window Website Opens in new window Participation in EU R&I programmes Opens in new window HORIZON collaboration network Opens in new window Total cost € 1 068 886,25 Participants (7) Sort alphabetically Sort by Net EU contribution Expand all Collapse all THE CHANCELLOR MASTERS AND SCHOLARS OF THE UNIVERSITY OF CAMBRIDGE United Kingdom Net EU contribution € 1 432 990,00 Address TRINITY LANE THE OLD SCHOOLS CB2 1TN Cambridge See on map Region East of England East Anglia Cambridgeshire CC Activity type Higher or Secondary Education Establishments Links Contact the organisation Opens in new window Website Opens in new window Participation in EU R&I programmes Opens in new window HORIZON collaboration network Opens in new window Total cost € 1 432 990,00 CELLENDES GMBH Germany Net EU contribution € 589 233,75 Address GERHARD KINDLER STRASSE 8 72770 REUTLINGEN See on map SME The organization defined itself as SME (small and medium-sized enterprise) at the time the Grant Agreement was signed. Yes Region Baden-Württemberg Tübingen Reutlingen Activity type Private for-profit entities (excluding Higher or Secondary Education Establishments) Links Contact the organisation Opens in new window Participation in EU R&I programmes Opens in new window HORIZON collaboration network Opens in new window Total cost € 589 233,75 FONDAZIONE IRCCS CA' GRANDA - OSPEDALE MAGGIORE POLICLINICO Italy Net EU contribution € 895 000,00 Address VIA FRANCESCO SFORZA 28 20122 Milano See on map Region Nord-Ovest Lombardia Milano Activity type Other Links Contact the organisation Opens in new window Website Opens in new window Participation in EU R&I programmes Opens in new window HORIZON collaboration network Opens in new window Total cost € 895 000,00 INSPHERO AG Switzerland Net EU contribution € 0,00 Address WAGISTRASSE 27 8952 Schlieren See on map SME The organization defined itself as SME (small and medium-sized enterprise) at the time the Grant Agreement was signed. Yes Region Schweiz/Suisse/Svizzera Zürich Zürich Activity type Private for-profit entities (excluding Higher or Secondary Education Establishments) Links Contact the organisation Opens in new window Website Opens in new window Participation in EU R&I programmes Opens in new window HORIZON collaboration network Opens in new window Total cost € 453 875,00 LONZA NETHERLANDS BV Netherlands Net EU contribution € 463 950,00 Address OXFORDLAAN 70 6229 EV Maastricht See on map Region Zuid-Nederland Limburg (NL) Zuid-Limburg Activity type Private for-profit entities (excluding Higher or Secondary Education Establishments) Links Contact the organisation Opens in new window Website Opens in new window Participation in EU R&I programmes Opens in new window HORIZON collaboration network Opens in new window Total cost € 463 950,00 SPARKS & CO Participation ended France Net EU contribution € 133 153,46 Address 66 AVENUE DES CHAMPS ELYSEES ESPACE 41 75008 Paris See on map SME The organization defined itself as SME (small and medium-sized enterprise) at the time the Grant Agreement was signed. Yes Region Ile-de-France Ile-de-France Paris Activity type Private for-profit entities (excluding Higher or Secondary Education Establishments) Links Contact the organisation Opens in new window Participation in EU R&I programmes Opens in new window HORIZON collaboration network Opens in new window Total cost € 133 153,46 PROSCIENCIA BERATUNGS-GMBH Germany Net EU contribution € 83 186,54 Address AUF DER HEIDE 11 23558 Luebeck See on map SME The organization defined itself as SME (small and medium-sized enterprise) at the time the Grant Agreement was signed. Yes Region Schleswig-Holstein Schleswig-Holstein Lübeck, Kreisfreie Stadt Activity type Private for-profit entities (excluding Higher or Secondary Education Establishments) Links Contact the organisation Opens in new window Participation in EU R&I programmes Opens in new window HORIZON collaboration network Opens in new window Total cost € 83 186,54