Objective
The immune system can be subdivided into two main arms: the innate and the adaptive. Recently, it was identified a new family of cells belonging to the innate system but that phenotypically and functionally resemble the specific subtypes of adaptive T lymphocytes. In mice and in humans, these innate lymphoid cells (ILCs) play critical roles in host defense, in shaping the microbiota and in tissue repair at mucosal tissues via secreting cytokines such as IL-22. The innate system is more ancient than the adaptive, however, the existence and diversity of ILCs in organisms other than mammals remains unknown. Also unknown is how ILCs interact with other cells within tissues. This project will address the existence and role of the different subsets of ILCs and the conservation of IL-22 function in a distant relative, the zebrafish, a genetically tractable vertebrate with remarkable optical accessibility.
To identify ILCs, cell populations likely enriched in these cells will be purified from mucosal sites such as the intestine and the gills by flow cytometry, guided by morphology and expression of reporter transgenes for immune cell types. Their transcriptome profile will be assessed in bulk and at the single-cell level adapting the microfluidic-based Biomark system to the zebrafish. Also, to know if cytokines produced by T cells can also be produced by non-adaptive lymphocytes – i.e. ILCs, as described in mouse, immune responses of zebrafish mutants lacking adaptive lymphocytes or all types of lymphocytes will be compared to that of wild-type fish.
To study IL-22 function, mutants for this cytokine and its receptor will be generated. Together with a transgenic il22 reporter line allowing the identification of IL-22-producing cells, they will be challenged with pathogens or inflammatory stimuli, and studied by various approaches including in vivo imaging. This work will allow a detailed in vivo study of the ontogeny, role and behavior of ILCs and IL-22- producing cells.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- medical and health sciences health sciences public health
- medical and health sciences medical biotechnology genetic engineering gene therapy
- medical and health sciences clinical medicine gastroenterology inflammatory bowel disease
- medical and health sciences clinical medicine emergency medicine graft versus host disease
- medical and health sciences basic medicine physiology homeostasis
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
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H2020-EU.1.3. - EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions
MAIN PROGRAMME
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H2020-EU.1.3.2. - Nurturing excellence by means of cross-border and cross-sector mobility
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Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
MSCA-IF-EF-ST - Standard EF
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Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
(opens in new window) H2020-MSCA-IF-2015
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Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.
75724 Paris
France
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.