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Directed Protein Evolution for Synthetic Biology and Biocatalysis

Directed Protein Evolution for Synthetic Biology and Biocatalysis

Objective

ES-Cat will use directed evolution as a tool to reproduce Nature's remarkable ability to generate molecular machines - in particular enzymes – that perform at levels near perfection. Instead of seeing rational and combinatorial approaches as alternatives, we combine them in this network to achieve a ‘smarter’ and more efficient exploration of protein
sequence space. By harnessing the forces of Darwinian evolution and design in the laboratory we want to (i) screen large and diverse libraries for proteins with improved and useful functions, (ii) optimize existing proteins for applications in medicine or biotechnology and (iii) provide a better understanding of how existing enzymes evolved and how
enzyme mechanisms can be manipulated. This Network brings together leading academic and industrial groups with diverse and complementary skills. The range of methodologies represented in ES-Cat allows an integrated approach combining in silico structural and sequence analysis with experimental high-throughput screening selection methods (phage-, ribozyme and SNAP display, robotic liquid handling, lab-on-a-chip/microfluidics) with subsequent systematic kinetic and biophysical
analysis. This integration of methods and disciplines will improve the likelihood of success of directed evolution campaigns, shorten biocatalyst development times, and make protein engineering applicable to a wider range of industrial targets. It will also train the next generation of creative researchers ready to fill roles in tailoring enzymes and other proteins for industrial application in synthetic biology efforts to move towards a bio-based economy, rivaling advances that are being made in the US and allowing the EU economy to harvest its evident socio-economic benefits.

Coordinator

THE CHANCELLOR MASTERS AND SCHOLARS OF THE UNIVERSITY OF CAMBRIDGE

Address

Trinity Lane The Old Schools
Cb2 1tn Cambridge

United Kingdom

Activity type

Higher or Secondary Education Establishments

EU Contribution

€ 273 287,88

Participants (9)

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UNIVERSITAET GREIFSWALD

Germany

EU Contribution

€ 498 432,96

FAKULTNI NEMOCNICE U SV. ANNY V BRNE

Czechia

EU Contribution

€ 232 422,48

WESTFAELISCHE WILHELMS-UNIVERSITAET MUENSTER

Germany

EU Contribution

€ 498 432,96

ENANTIS SRO

Czechia

EU Contribution

€ 232 422,48

RIJKSUNIVERSITEIT GRONINGEN

Netherlands

EU Contribution

€ 510 748,56

BEN-GURION UNIVERSITY OF THE NEGEV

Israel

EU Contribution

€ 260 300,52

MEDIMMUNE LIMITED

United Kingdom

EU Contribution

€ 546 575,76

JOHNSON MATTHEY PLC

United Kingdom

EU Contribution

€ 273 287,88

UNIVERSITE CATHOLIQUE DE LOUVAIN

Belgium

EU Contribution

€ 501 120

Partners (9)

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DROP-TECH LTD

ENZYMICALS AG

GLAXOSMITHKLINE RESEARCH AND DEVELOPMENT LTD.

Kamada

DSM FOOD SPECIALTIES BV

CAMBRIDGE ENTREPRISE LIMITED

Alzheimer's Research UK

NOVOZYMES A/S

Brain AG

Project information

Grant agreement ID: 722610

Status

Ongoing project

  • Start date

    1 March 2017

  • End date

    28 February 2021

Funded under:

H2020-EU.1.3.1.

  • Overall budget:

    € 3 827 031,48

  • EU contribution

    € 3 827 031,48

Coordinated by:

THE CHANCELLOR MASTERS AND SCHOLARS OF THE UNIVERSITY OF CAMBRIDGE

United Kingdom