The nanoparticle-scope : a new integrated instrument for accurate and reproducible physico-chemical characterisation of nanoparticles (npSCOPE)

Deliverables

Documents, reports (8)

Nano-material sampling and preparation protocols

Guide describing the preparation, handling and storage of the nano-material samples

Evaluation report on performance of the npSCOPE instrument in representative test cases for risk assessment

Presentation and evaluation of npSCOPE results obtained in the nanotoxicology test cases, including reproducibility based on the round-robin studies and benchmarking against existing state-of-the-art instruments.

Description of correlative workflow

A description of the sequence of measurements to be followed to ensure adequate characterisation (both for nano-toxicology studies and other applications of the npSCOPE instrument)

Evaluation report on performance of the npSCOPE instrument for applications beyond nanotoxicology

Presentation and evaluation of npSCOPE results obtained in test examples in materials-science, nanomedicine and pharmaceutics, including reproducibility based on the round-robin studies and benchmarking against existing state-of-the-art instruments.

List of validated performance specifications of the npSCOPE instrument

List of performance specifications of the instrument with details about samples and protocols used to determine the various parameters.

Global validation report of npSCOPE instrument, including benchmarking against existing state-of-the-art techniques

Report describing the overall performance of the npSCOPE instrument and methodologies, the application landscape and roadmaps for its future use.

Confirmed specification

List of performance specifications for the npSCOPE instrument and its sub-systems, including mechanical and electrical interface definitions

Description of cryo-workflow

A description of the sequence of measurements to be followed (sample preparation and characterisation workflows) to ensure adequate characterisation (both for nano-toxicology studies and other applications of the npSCOPE instrument) under cryo-conditions

Websites, patent fillings, videos etc. (1)

Project website

Website for the project is publically accessible with preliminary content

Other (1)

Final version of the software for data processing

A final version of the data analysis software for the automated data processing and reporting within the npSCOPE project is delivered, focusing on datasets from the npSCOPE instrument

Publications

Peer reviewed articles (20)

Scanning transmission imaging in the helium ion microscope using a microchannel plate with a delay line detector

Author(s): Eduardo Serralta, Nico Klingner, Olivier De Castro, Michael Mousley, Santhana Eswara, Serge Duarte Pinto, Tom Wirtz, Gregor Hlawacek
Published in: Beilstein Journal of Nanotechnology, 11, 2020, Page(s) 1854-1864, ISSN 2190-4286
Publisher: Beilstein-Institut Zur Forderung der Chemischen Wissenschaften
DOI: 10.3762/bjnano.11.167

Critical Assessment of Analytical Methods for the Harmonized and Cost-Efficient Analysis of Microplastics

Author(s): Sebastian Primpke, Silke H. Christiansen, Win Cowger, Hannah De Frond, Ashok Deshpande, Marten Fischer, Erika B. Holland, Michaela Meyns, Bridget A. O'Donnell, Barbara E. Ossmann, Marco Pittroff, George Sarau, Barbara M. Scholz-Böttcher, Kara J. Wiggin
Published in: Applied Spectroscopy, 74/9, 2020, Page(s) 1012-1047, ISSN 0003-7028
Publisher: Society for Applied Spectroscopy
DOI: 10.1177/0003702820921465

Supported Two-Dimensional Materials under Ion Irradiation: The Substrate Governs Defect Production

Author(s): Silvan Kretschmer, Mikhail Maslov, Sadegh Ghaderzadeh, Mahdi Ghorbani-Asl, Gregor Hlawacek, Arkady V. Krasheninnikov
Published in: ACS Applied Materials & Interfaces, 10/36, 2018, Page(s) 30827-30836, ISSN 1944-8244
Publisher: American Chemical Society
DOI: 10.1021/acsami.8b08471

Stationary beam full-field transmission helium ion microscopy using sub-50 keV He + : Projected images and intensity patterns

Author(s): Michael Mousley, Santhana Eswara, Olivier De Castro, Olivier Bouton, Nico Klingner, Christoph T Koch, Gregor Hlawacek, Tom Wirtz
Published in: Beilstein Journal of Nanotechnology, 10, 2019, Page(s) 1648-1657, ISSN 2190-4286
Publisher: Beilstein-Institut Zur Forderung der Chemischen Wissenschaften
DOI: 10.3762/bjnano.10.160

Highest resolution chemical imaging based on Secondary Ion Mass Spectrometry performed on the Helium Ion Microscope

Author(s): Jean-Nicolas Audinot, Patrick Philipp, Olivier De Castro, Antje Biesemeier, Hung Quang Hoang, Tom Wirtz
Published in: Reports on Progress in Physics, 2021, ISSN 0034-4885
Publisher: Institute of Physics Publishing
DOI: 10.1088/1361-6633/ac1e32

Freestanding and Supported MoS 2 Monolayers under Cluster Irradiation: Insights from Molecular Dynamics Simulations

Author(s): Sadegh Ghaderzadeh, Vladimir Ladygin, Mahdi Ghorbani-Asl, Gregor Hlawacek, Marika Schleberger, Arkady V. Krasheninnikov
Published in: ACS Applied Materials & Interfaces, 12/33, 2020, Page(s) 37454-37463, ISSN 1944-8244
Publisher: American Chemical Society
DOI: 10.1021/acsami.0c09255

Correlative Electron Microscopy, High Resolution Ion Imaging and Secondary Ion Mass Spectrometry for High Resolution Nanoanalytics on Biological Tissue

Author(s): Antje Biesemeier, Olivier De Castro, Eduardo Serralta, Jelena Lovric, Santhana Eswara, Jean-Nicolas Audinot, Sébastien Cambier, Tom Wirtz
Published in: Microscopy and Microanalysis, 26/S2, 2020, Page(s) 818-820, ISSN 1431-9276
Publisher: Cambridge University Press
DOI: 10.1017/s1431927620015950

Critical Review of Processing and Classification Techniques for Images and Spectra in Microplastic Research

Author(s): Win Cowger, Andrew Gray, Silke H. Christiansen, Hannah DeFrond, Ashok D. Deshpande, Ludovic Hemabessiere, Eunah Lee, Leonid Mill, Keenan Munno, Barbara E. Ossmann, Marco Pittroff, Chelsea Rochman, George Sarau, Shannon Tarby, Sebastian Primpke
Published in: Applied Spectroscopy, 74/9, 2020, Page(s) 989-1010, ISSN 0003-7028
Publisher: Society for Applied Spectroscopy
DOI: 10.1177/0003702820929064

Magnetic Sector SIMS System with Continuous Focal Plane Detector for Advanced Analytical Capabilities on FIB Instruments

Author(s): Tom Wirtz, Olivier De Castro, Antje Biesemeier, Hung Quang Hoang, Jean-Nicolas Audinot
Published in: Microscopy and Microanalysis, 26/S2, 2020, Page(s) 1972-1974, ISSN 1431-9276
Publisher: Cambridge University Press
DOI: 10.1017/s1431927620019984

Correlative Microscopy and Spectroscopy Workflow for Microplastics

Author(s): George Sarau, Lasse Kling, Barbara E. Oßmann, Ann-Katrin Unger, Frank Vogler, Silke H. Christiansen
Published in: Applied Spectroscopy, 74/9, 2020, Page(s) 1155-1160, ISSN 0003-7028
Publisher: Society for Applied Spectroscopy
DOI: 10.1177/0003702820916250

Channeling effects in gold nanoclusters under He ion irradiation: insights from molecular dynamics simulations

Author(s): Sadegh Ghaderzadeh, Mahdi Ghorbani-Asl, Silvan Kretschmer, Gregor Hlawacek, Arkady V Krasheninnikov
Published in: Nanotechnology, 31/3, 2020, Page(s) 035302, ISSN 0957-4484
Publisher: Institute of Physics Publishing
DOI: 10.1088/1361-6528/ab4847

Advanced Analytical Capabilities on FIB Instruments Using SIMS

Author(s): Tom Wirtz, Olivier De Castro, Antje Biesemeier, Hung Quang Hoang, Jean-Nicolas Audinot
Published in: Microscopy and Microanalysis, 26/S2, 2020, Page(s) 82-83, ISSN 1431-9276
Publisher: Cambridge University Press
DOI: 10.1017/s143192762001332x

Imaging and Analytics on the Helium Ion Microscope

Author(s): Tom Wirtz, Olivier De Castro, Jean-Nicolas Audinot, Patrick Philipp
Published in: Annual Review of Analytical Chemistry, 12/1, 2019, Page(s) 523-543, ISSN 1936-1327
Publisher: Annual Reviews, Inc.
DOI: 10.1146/annurev-anchem-061318-115457

In vitro exposure of a 3D-tetraculture representative for the alveolar barrier at the air-liquid interface to silver particles and nanowires

Author(s): Ionel Fizeșan, Sébastien Cambier, Elisa Moschini, Aline Chary, Inge Nelissen, Johanna Ziebel, Jean-Nicolas Audinot, Tom Wirtz, Marcin Kruszewski, Anca Pop, Béla Kiss, Tommaso Serchi, Felicia Loghin, Arno C. Gutleb
Published in: Particle and Fibre Toxicology, 16/1, 2019, ISSN 1743-8977
Publisher: BioMed Central
DOI: 10.1186/s12989-019-0297-1

Synthetic Image Rendering Solves Annotation Problem in Deep Learning Nanoparticle Segmentation

Author(s): Leonid Mill, David Wolff, Nele Gerrits, Patrick Philipp, Lasse Kling, Florian Vollnhals, Andrew Ignatenko, Christian Jaremenko, Yixing Huang, Olivier De Castro, Jean‐Nicolas Audinot, Inge Nelissen, Tom Wirtz, Andreas Maier, Silke Christiansen
Published in: Small Methods, 5/7, 2021, Page(s) 2100223, ISSN 2366-9608
Publisher: Wiley-VCH GmbH
DOI: 10.1002/smtd.202100223

In situ Correlative Helium Ion Microscopy and Secondary Ion Mass Spectrometry for High-Resolution Nano-Analytics in Life Sciences

Author(s): Jelena Lovric, Jean-Nicolas Audinot, Tom Wirtz
Published in: Microscopy and Microanalysis, 25/S2, 2019, Page(s) 1026-1027, ISSN 1431-9276
Publisher: Cambridge University Press
DOI: 10.1017/s1431927619005865

Structural and chemical evolution of Au-silica core–shell nanoparticles during 20 keV helium ion irradiation: a comparison between experiment and simulation

Author(s): M. Mousley, W. Möller, P. Philipp, G. Hlawacek, T. Wirtz, S. Eswara
Published in: Scientific Reports, 10/1, 2020, Page(s) 12058, ISSN 2045-2322
Publisher: Nature Publishing Group
DOI: 10.1038/s41598-020-68955-7

npSCOPE: A New Instrument Combining SIMS Imaging, SE Imaging and Transmission Ion Microscopy for High Resolution In-situ Correlative Investigations

Author(s): Olivier De Castro, Antje Biesemeier, Eduardo Serralta, Nico Klingner, Gregor Hlawacek, Peter Gnauck, Serge Duarte Pinto, Falk Lucas, Cecilia Bebeacua, Tom Wirtz
Published in: Microscopy and Microanalysis, 26/S2, 2020, Page(s) 1976-1977, ISSN 1431-9276
Publisher: Cambridge University Press
DOI: 10.1017/s1431927620019996

Novel diagnostic and therapeutic techniques reveal changed metabolic profiles in recurrent focal segmental glomerulosclerosis

Author(s): Janina Müller-Deile, George Sarau, Ahmed M. Kotb, Christian Jaremenko, Ulrike E. Rolle-Kampczyk, Christoph Daniel, Stefan Kalkhof, Silke H. Christiansen, Mario Schiffer
Published in: Scientific Reports, 11/1, 2021, Page(s) 1, ISSN 2045-2322
Publisher: Nature Publishing Group
DOI: 10.1038/s41598-021-83883-w

Jejunal villus absorption and paracellular tight junction permeability are major routes for early intestinal uptake of food-grade TiO2 particles: an in vivo and ex vivo study in mice

Author(s): Christine Coméra, Christel Cartier, Eric Gaultier, Olivier Catrice, Quentin Panouille, Sarah El Hamdi, Kristof Tirez, Inge Nelissen, Vassilia Théodorou, Eric Houdeau
Published in: Particle and Fibre Toxicology, 17/1, 2020, Page(s) 26, ISSN 1743-8977
Publisher: BioMed Central
DOI: 10.1186/s12989-020-00357-z